急性乳腺炎不同中医证型与IL-6、IL-10和TNF-α的相关性研究

A Study on Correlation between Different Chinese Medicine Syndrome Types of Acute Mastitis and IL-6,IL-10 and TNF-α

目的:观察不同中医证型急性乳腺炎患者与外周血清中白细胞介素-6 (IL-6)、白细胞介素-10 (IL-10)及肿瘤坏死因子-α(TNF-α)表达的差异。方法:采用ELISA法检测103例不同中医证型急性乳腺炎患者外周血清IL-6、IL-10和TNF-α的表达水平,并与对照组18例健康体验者进行比较。结果:103例急性乳腺炎患者中医证型中,热毒炽盛证最多,其次为正虚毒恋证,而气滞热壅证最少。与对照组比较,急性乳腺炎组IL-6、IL-10及TNF-α均升高,差异均有统计学意义(P<0.05)。在103例急性乳腺炎组患者的证型分组中,热毒炽盛证、气滞热壅证、正虚毒恋证各组的IL-6表达水平比对照组高,差异均有统计学意义(P<0.05);热毒炽盛证、气滞热壅证、正虚毒恋证各组的IL-10、TNF-α表达水平均比对照组高,差异均有统计学意义(P<0.01);热毒炽盛证组的IL-6、IL-10和TNF-α的表达水平与气滞热壅证组、正虚毒恋证组比较,差异均有统计学意义(P<0.05)。结论:IL-6、IL-10及TNF-α水平可作为急性乳腺炎中医辨证分型的客观指标,为急性乳腺炎的中医辨证治疗提供可量化的参考指标。

Objective:To observe the differences between different Chinese medicine syndrome types of acute mastitis and the expressions of interleukin-6(IL-6),interleukin-10(IL-10) and tumor necrosis factor-α(TNF-α) in serum in peripheral blood.Methods:The levels of expressions of IL-6,IL-10 and TNF-α in serum in peripheral blood of 103 patients with acute mastitis of different Chinese medicine syndrome types were detected by the ELISA method,and were compared with those of healthy objects in the control group.Results:In the Chinese medicine syndrome types of 103 cases of patients with acute mastitis,the most common syndrome type is intense heat toxin syndrome,followed by deficient healthy qi leading to lingering toxin syndrome,and the qi stagnation and heat obstruction syndrome is the least.Compared with those in the control group,the levels of expressions of IL-6,IL-10 and TNF-α in the acute mastitis group were increased,differences being significant(P<0.05).The acute mastitis group was divided into three groups according to their syndrome types;the levels of expression of IL-6 in the intense heat toxin syndrome group,the qi stagnation and heat obstruction syndrome group and the deficient healthy qi leading to lingering toxin syndrome group were higher than those in the control group respectively,differences being significant(P<0.05);the levels of expressions of IL-10 and TNF-α in the three groups were higher than those in the control group respectively,differences being significant(P<0.01);when compared the levels of expressions of IL-6,IL-10 and TNF-α in the intense heat toxin syndrome group with those in the qi stagnation and heat obstruction syndrome group and the deficient healthy qi leading to lingering toxin syndrome group,the difference was significant(P<0.05).Conclusion:In syndrome differentiation and treatment of Chinese medicine of acute mastitis,the levels of IL-6,IL-10 and TNF-α can be used as objective indicators and quantitative indicators for reference.