甲状腺乳头状癌p53基因及蛋白的表达情况及临床意义

Expression of p53 gene and protein in papillary thyroid carcinoma and its clinical significance

目的研究甲状腺乳头状癌(PTC)p53基因及蛋白的表达情况及临床意义。方法选择143例该院住院手术切除的PTC石蜡标本,另外收集同期因甲状腺良性病变切除的正常甲状腺组织标本68例。分别采用免疫组化法及western blot法检测PTC组织和正常甲状腺组织中p53蛋白的表达情况;采用实时荧光定量聚合酶链反应(RT-PCR)测定PTC组织和正常甲状腺组织中p53 mRNA相对表达量;探讨p53蛋白表达与PTC临床病理特征的关系;采用Kaplan-Meier法进行生存分析,分析p53蛋白表达与PTC患者预后的关系。结果免疫组化结果显示,p53蛋白在PTC组织中呈阳性表达;RT-PCR结果显示,PTC组织中的p53 mRNA表达高于正常甲状腺组织(P<0.05);western blot结果显示,p53蛋白在PTC组织中表达水平明显高于正常甲状腺组织(P<0.05)。PTC组织中p53蛋白的表达水平与性别、年龄、肿瘤直径、肿瘤分化程度无关(P>0.05),而与TNM分期、淋巴结转移有关(P<0.05)。Kaplan-Meier法生存分析显示,p53蛋白阳性表达组5年生存率明显低于p53蛋白阴性表达组(P<0.05)。结论p53基因及蛋白在PTC中大多表达上调,参与PTC的发生、发展,影响患者预后,值得临床重视。

Objective To study the expression of p53 gene and protein in papillary thyroid carcinoma(PTC)and its clinical significance.Methods A total of 143 resected PTC paraffin specimens were collected from the hospitalized patients,and other contemporaneous 68 normal thyroid tissue specimens were collected from the resected thyroid benign lesions.The expression levels of p53 protein in PTC tissues and normal thyroid tissues were detected by adopting the immunohistochemistry(IHC)and western blot respectively.The real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to determine the relative expression levels of p53 mRNA in PTC tissues and normal thyroid tissues.The relationship between the expression level of p53 protein and clinicopathological characteristics of PTC was investigated.The Kaplan-meier method was used for conducting the survival analysis.The relationship between the p53 protein expression with prognosis and survival of PTC patients was analyzed.Results The IHC results showed that p53 protein was positively expressed in PTC tissues;the RT-PCR results showed that the p53 mRNA expression in the PTC tissue was higher than that in the normal thyroid tissue(P<0.05);the western blot results showed that the p53 protein expression level in the PTC tissue was significantly higher than that in the normal thyroid tissue(P<0.05).The p53 protein level in the PTC tissue had no relationship with the sex,age,tumor diameter and tumor differentiation(P>0.05),while had the relationship with the TNM classification and lymph node metastasis(P<0.05).The Kaplan-meier survival analysis showed that the 5-year survival rate in the p53 positive expression group was significantly lower than that in the p53 negative expression group(P<0.05).Conclusion p53 protein is mostly up-regulated in PTC,which may be involved in the occurrence,development and prognosis of PTC,and is worthy of clinical attention.