掌叶梁王茶无菌扦插和愈伤组织诱导

Sterile Cuttage and Callus Induction of Nothopanax delavayi

为进一步促进掌叶梁王茶无菌快繁和生产利用,以一年生具有腋芽的掌叶梁王茶枝条为无菌扦插材料,用0.1%HgCl 2对其消毒不同时间,接入KT和NAA不同浓度配比的MS培养基中,观察其染菌率、黄化率以及诱导率。以无菌扦插所获无菌苗的叶片作为外植体,接入NAA与6-BA或TDZ不同激素配比的MS培养基中,观察其出愈率。研究结果表明:消毒时间为11 min时,染菌率为30.28%,黄化率为40.35%,掌叶梁王茶茎段消毒效果最好。NAA和KT药剂对茎段诱导无菌苗均有影响,KT药剂影响达极显著水平,NAA药剂影响达显著水平,两因子间无互作效应,因子影响顺序为KT>NAA。用0.10 mg·L^-1 NAA+0.50 mg·L^-1 KT培养基培养,茎段的诱导率最高,达100%。无菌叶片在0.25 mg·L^-1 NAA+1.00 mg·L^-1 TDZ培养基中诱导,愈伤组织效果最佳,出愈率达93%(P<0.05)。

In order to promote further aseptic and rapid propagation and production and utilization of Nothopanax delavayi.The bacteria were disinfected with 0.1%HgCl 2 at different times,and were put into MS medium with different concentration ratios of KT and NAA,and the infection rate,yeloration rate and induction rate were observed.The leaves of sterile seedlings obtained from aseptic cuttings were used as explants.The recovery rate was observed in MS culture medium with different ratios of NAA and 6-BA or TDZ hormones.The results showed that when the disinfection time was 11 min,the bacterial infection rate was 30.28%and the yellowing rate was 40.35%,the best disinfection effect is on stem segment of N.delavayi.Both NAA and KT agents had an effect on stem segment induced no-virus seedlings.KT agents had an extremely significant effect,and NAA agents had a significant effect.There was no interaction effect between the two factors,and the order of factor influence was KT>NAA.When cultured with 0.10 mg·L^-1 NAA+0.50 mg·L^-1 KT medium,the induction rate of stem segment was the highest,reaching 100%.Sterile blades were induced in 0.25 mg·L^-1 NAA+1.00 mg·L^-1 TDZ medium,and the callus effect was the best,with a recovery rate of 93%(P<0.05).