摘要
目的:竹叶碳苷黄酮在胃肠道消化过程中易于降解,导致其生物可及性低,因此,本研究拟探究黄原胶存在时,4种碳苷黄酮(异荭草苷、荭草苷、异牡荆素、牡荆素)在各模拟消化阶段生物可及性的变化情况。方法:利用体外模拟消化模型结合超高效液相色谱技术,分析在有/无黄原胶存在的条件下,竹叶提取物中4种碳苷黄酮质量浓度的变化情况。结果:相较于直接消化样品,添加黄原胶的样品可以明显减弱模拟消化过程中碳苷黄酮的降解;口腔、胃、肠道模拟消化对竹叶碳苷黄酮的生物可及性影响存在差异,黄原胶的加入可以明显提高4种碳苷黄酮,尤其是牡荆素和异牡荆素的生物可及性,模拟肠道消化后,黄原胶保护组的异牡荆素和牡荆素质量浓度分别降低了62%和59%,远低于直接消化组(分别降低84%和80%)。结论:黄原胶可以明显提升体外模拟消化过程中竹叶碳苷黄酮的生物可及性,对于相关功能产品的开发具有良好的应用价值。
Objective: Flavone C-glycosides of bamboo leaves are susceptible to degradation during gastrointestinal digestion, leading to low bioaccessibility. Given this background, this study evaluated the changes in the bioaccessiblity of four flavone C-glycosides(orientin, isoorientin, vitexin, and isovitexin) present in the 80% ethanol extract of bamboo leaves at various stages of simulated gastrointestinal digestion in the presence of xanthan gum. Methods: Simulated digestion was carried out in the presence and absence of xanthan gum. The changes in flavone C-glycoside concentrations were analyzed by ultra-high performance liquid chromatography(UPLC). Results: Addition of xanthan gum significantly attenuated the degradation of flavone C-glycosides during the digestion process. The oral, gastric and intestinal phases had different impacts on the bioaccessibility of flavone C-glycosides. Xanthan gum could significantly enhance the bioaccessibility of the four flavonoid C-glycosides, especially vitexin and isovitexin. The concentrations of isovitexin and vitexin in the xanthan gum group decrease by 62% and 59% after intestinal digestion, respectively, which were much lower than those(decrease by 84% and 80%) observed in the presence of xanthan gum. Conclusion: Xanthan gum can significantly improve the bioaccessibility of bamboo leaf flavone C-glycosides during simulated in vitro digestion, suggesting promising applications for the development of functional products.
作者
李占明
蔡泓历
梁奕
毛豪
廖晗雪
俞玥
周冬仁
LI Zhanming;CAI Hongli;LIANG Yi;MAO Hao;LIAO Hanxue;YU Yue;ZHOU Dongren(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Food Science Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Key Laboratory of Fish Health and Nutrition of Zhejiang Province,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第21期58-64,共7页
Food Science
基金
浙江省淡水水产研究所开放课题(ZJK201912
ZJK201805)
浙江省自然科学基金项目(LQ17C200002)
浙江省教育厅科研项目(Y201840401)
湖州市公益性研究项目(2018GZ28)
关键词
黄酮
超高效液相色谱
体外模拟胃肠道消化
生物可及性
黄原胶
flavonoids
ultra-high performance liquid chromatography
simulated in vitro gastrointestinal digestion
bioaccessibility
xanthan gum