邻苯二甲酸二丁酯的光敏毒性作用及机制

Photoinduced Toxicity and Mechanism of Action of Dibutyl Phthalate

天然邻苯二甲酸二丁酯(dibutyl phthalate,DBP)普遍存在于食药用胶质菌中,其潜在危害已逐渐引起人们的重视,因此,本研究探讨DBP的光敏毒性作用及机制。通过对雄性大鼠灌胃染毒(2 000 mg/kg mb DBP)并利用高效液相色谱检测DBP在大鼠体内分布和代谢情况;此外,检测大鼠肝脏和肾脏组织细胞的活性氧(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)含量和超氧化物歧化酶(superoxide dismutase,SOD)活力变化;同时,对大鼠肝脏和肾脏组织的病理切片进行观察。药代动力学实验发现日光能促进DBP转化为邻苯二甲酸单丁酯(mono-n-butylphthalate,MBP),其中肝脏和肾脏中MBP含量高于其他器官。MBP可以明显升高大鼠肝脏和肾脏中ROS和MDA含量,降低GSH含量和SOD活力,表明MBP会诱导大鼠肝脏发生脂质过氧化反应,引起早期的肝脏损伤。病理切片表明光照能增强MBP对大鼠肝脏和肾脏细胞的损伤程度。结论:DBP是光敏毒性物质,其在食品及包装材料等方面的应用应引起高度重视。

Dibutyl phthalate(DBP) is naturally occurs ubiquitously in medicinal and edible jelly fungi, and its potential harm to the environment and organisms has increasingly become a major concern. In this study, the photoinduced toxicity of DBP was studied and its mechanism of action was investigated. The metabolism and distribution of DBP in the plasma and liver of rats intragastrically administered with it were detected by high performance liquid chromatography(HPLC). Moreover, the contents of reactive oxygen species(ROS), malondialdehyde(MDA) and glutathione(GSH) and superoxide dismutase(SOD) activity in rat liver and kidney were determined, and the pathological sections of liver and kidney were observed. Pharmacokinetics showed that sunlight could promote the transformation of DBP into mono-n-butylphthalate(MBP), and MBP content in the liver and kidney was higher than that in other organs. MBP could significantly increase ROS and MDA levels and decrease GSH level and SOD activity in rat liver and kidney, providing evidence that MBP could induce lipid peroxidation in rat liver and induce early liver injury. The pathological sections showed that light could enhance the damage of MBP to rat liver and kidney cells. Therefore, DBP is a potentially harmful phototoxic substance in male rats. In conclusion, much attention should be paid to its application in foods and packaging materials.