利用二甲戊灵诱导玉米孤雌生殖的研究

Study on Maize Parthenogenesis Induced by Pendimethalin

以3种遗传类型不同的玉米杂交种先玉335 (普通型)、晋鲜糯6号(糯质型)和迪甜10号(甜质型)为试验材料,以蒸馏水处理为对照,二甲戊灵诱导液浓度设50、75、100、150 umol/L计5个处理,研究了不同浓度二甲戊灵对玉米孤雌生殖诱导率的影响。结果表明:3种类型玉米的孤雌生殖结实株率均随二甲戊灵浓度的升高呈先增加后降低的变化趋势,但不同类型玉米适宜的药剂浓度存在一定差异,先玉335、晋鲜糯6号、迪甜10号适宜的二甲戊灵处理浓度分别为100、100和75μmol/L。并对诱导后的孤雌生殖1代植株进行田间观察和鉴定,伪株比例为57.45%,产生的原因可能与其他无融合生殖途径及细胞质有关。

Taking three maize hybrids with different genetic types(Xianyu 335,Jinxiannuo No.6 and Ditian No.10)as the material,taking distilled water as the control,the concentration of pendimethalin was set at 50,75,100,125 and 150 umol/L for 5 treatments,the influence of different concentration of pendimethalin on the parthenogenesis induction rate of different kinds of maize were studied.The results showed that parthenogenesis seeding rate of the three maize increased first and then decreased with the increasing of concentration of pendimethalin,and the suitable concentration of pendimethalin for Xianyu 335,Jinxiannuo No.6 and Ditian No.10 was 100、100和75μmol/L,respectively.The first generation of treating maize were observed and identified in the field to verify the induction effect,the ratio of false homozygous plants was 57.45%and the causes of abnormal plants were analyzed.