Cervista高危HPV分型与CK8/18、P120ctn表达在预测低级别鳞状上皮内病变进展和转归中的价值

The value of Cervista high risk HPV and CK8/18, P120ctn expression in predicting the progress and outcome of low grade squamous intraepithelial lesion

目的探讨Cervista高危人乳头瘤病毒(HR-HPV)分型与CK8/18、P120ctn在预测低级别鳞状上皮内病变(LSIL)进展和转归中的价值。方法收集2012年12月~2016年12月就诊于南方医科大学附属佛山妇幼保健院同时行TCT及组织活检确诊为LSIL患者119例,其中进展组28例,持续组41例,消退组50例。另收集HSIL宫颈鳞癌(SCC)对照组20例,正常对照组15例。采用Cervista HR-HPV分型方法检测各组HR-HPV分型,运用Ventana全自动免疫组化法检测各组CK8/18、P120ctn表达情况。结果进展组A9型阳性率均高于A7及A5/A6型,高于持续组及消退组,LSIL组间HR-HPV分型比较,差异有统计学意义(P<0.05);CK8/18阳性表达率进展组为67.9%、持续组为41.5%、消退组为20.0%;LSIL组分别与HSIL\SCC对照组、正常对照组比较,差异有统计学意义(P<0.05);P120ctn阳性表达率进展组为82.1%、持续组为90.2%、消退组为100.0%;LSIL组P120ctn阳性表达率低于正常对照组,高于HSIL\SCC对照组,差异有统计学意义(P<0.05)。结论联合检测Cervista HR-HPV分型及CK8/18、P120ctn表达对预测LSIL进展和转归有一定价值。

Objective To investigate Cervista high risk HPV(HR-HPV)typing and the value of CK8/18 and P120ctn in predicting the progression and prognosis of low-grade squamous intraepithelial lesions(LSIL).Methods Atotal of 119 patients with LSIL admitted to Foshan Women and Children Hospital Affiliated to Southern Medical University from December 2012 to December 2016 were selected as the subjects.There were progressive group(28 cases),persistent group(41 cases)and regression group(50 cases).In addition,20 patients in the HSIL\squamous cell carcinoma(SCC)control group and 15 patients in the normal cervical were collected as normal control group.The HR-HPV genotypes were detected by Cervista and then the expression of CK8/18 and P120ctn were detected by Ventana full automatic immunohistochemical.Results The positive rates of A9 in the progressive group were higher than those of A7 and A5/A6.The positive rates of A9 in progressive group were higher than persistent group and regression group.It was significant difference in HR-HPV typing among LSIL groups(P<0.05).The positive expression rate of CK8/18 was 67.9%in the progressive group,was 41.5%in the persistent group and was 20.0% in the regressive group.The positive expression rate of CK8/18 in the LSIL group was significantly higher than that in the HSIL\SCC control group and normal control groups(P<0.05).The positive expression rate of P120ctn was 82.1%in the progressive group,was 90.2%in the persistent group and was 100.0% in the regressive group.The positive expression rate of P120ctn in LSIL group was lower than that in the normal control group,the LSIL group was higher than the HSIL\SCC control group,the differences were statistically significant(all P<0.05).Conclusion Simultaneous testing Cervista HR-HPV typing and expression of CK8/18 and P120ctn may be useful in predicting the progression and prognosis of LSIL.