天麻基因组DNA不同提取方法比较

Comparison of Different Extraction Methods of Genome DNA in Gastrodia elata B1.

以天麻块茎为供试材料,采用琼脂糖凝胶电泳检测和紫外分光光度含量测定技术,比较改良CTAB法和SDS-CTAB法提取天麻块茎基因组DNA的效果。结果表明,改良CTAB法提取天麻块茎基因组DNA的产量高于SDS-CTAB法,纯度略次于SDS-CTAB法,二者提取的基因组DNA,DNA结构完整、大片段的DNA分子含量高。该研究为天麻基因组DNA提取方法的选择提供了参考依据,为天麻种质资源的分子遗传研究奠定了一定的科学与技术基础。

Taking Gastrodia elata B1.tuber as material,agarose gel electrophoresis and ultraviolet spectrophotometry were used to compare the effects of improved CTAB and SDS-CTAB on genomic DNA from rhizoma tuber of G.elata.The results showed that the yield of genomic DNA extracted from G.elata tubers by improved CTAB method was higher than that by SDS-CTAB method,and the purity was slightly lower than that by SDS-CTAB method.The results provided a reference for selecting genomic DNA extraction methods of G.elata and laid a scientific and technical foundation for the molecular genetic research of G.elata germplasm resources.