鱼腥草中总黄酮的提取工艺优化研究

Optimized procedure to extract the total flavonoids from Houstonian cordata

以鱼腥草(Houttuynia cordata Thunb.)总黄酮提取率为考察指标,以芦丁为对照品,在单因素试验的基础上,采用正交设计对乙醇体积分数、料液比、提取温度及提取时间4因素进行优选。结果表明,将鱼腥草粉末与70%乙醇溶液进行1∶25混合,置于70℃恒温水浴箱中浸提90 min,此条件下鱼腥草总黄酮提取率为3.640%,为优化鱼腥草中总黄酮的提取工艺提供了参考依据。

The extraction medium was selected,the extraction rate of total flavonoids from Houttuynia cordata was taken as the index,and rutin was taken as the reference substance.On the basis of single factor test,four factors such as ethanol concentration,liquid-liquid ratio,extraction temperature and extraction time were optimized by orthogonal design.The results showed that the optimal solution for extracting total flavonoids from Houttuynia cordata by ethanol extraction method was as follows:Houttuynia cordata powder was mixed with 75%ethanol solution in a ratio of 1∶25 and placed in a constant temperature water bath at 70℃ for 90 min,the total flavonoid extraction rate of Houttuynia cordata was 3.640%.This experiment provides a reference for optimizing the extraction process of total flavonoids from Houttuynia cordata.