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不同厂家猪血清配制的培养基培养猪肺炎支原体的比较研究 被引量:3

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摘要 根据培养物的生长速度和含菌量方面筛选适合猪肺炎支原体CJ株生长的猪血清。配制改良Friis培养基时分别添加6种不同生产厂家的猪血清,由6种不同厂家猪血清配制的改良Friis培养基培养猪肺炎支原体CJ株。从生长时间可知,由厂家1的猪血清配制的改良Friis培养基更适宜猪肺炎支原体CJ株的生长。从测定的含菌量测定结果可知,由厂家1的猪血清配制的改良Friis培养基培养猪肺炎支原体CJ株的含菌量较高。结果表明,由厂家1的猪血清配制的改良Friis培养基培养猪肺炎支原体CJ株的生长时间短且含菌量高。
出处 《甘肃畜牧兽医》 2019年第9期66-68,72,共4页 Gansu Animal Husbandry and Veterinary Medicine
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  • 1沈青春,覃青松,王琴,宁宜宝,赵耘,宋立,张广川,吴惠明.PCR方法测定猪肺炎支原体培养物菌数[J].中国预防兽医学报,2006,28(1):55-57. 被引量:10
  • 2南京农业大学.家畜传染病学[M].北京:农业出版社,1980..
  • 3Leon EA, Madec F, Taylor NM, et al. Seroepidemiology of Mycoplasma hyopneumoniae in pigs from farrow-to-finish farms[J]. Vet Microbiol, 2001,78(4): 331-341.
  • 4Freeman MJ, Lopez-Osuna M, Armstrong CH, et al. Evaluation of the indirect hemagglutination assay as a practical serodiagnostic test for Mycoplasmal pneumonia of swine[J]. Vet Microbiol, 1984, 9(3) : 259-270.
  • 5Bereiter M, Young TF, Joo HS, et al. Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum[J]. Vet Microbiol, 1990,25(2-3) : 177-192.
  • 6Artiushin S, Stipkovits L, Minion FC. Development of polymerase chain reaction primers to detect Mycoplasma hyopneumoniae [ J ].Mol Cell Probes, 1993, 7(5) : 381-385.
  • 7Stemke GW. Gene amplification (PCR) to detect and differentiate Mycoplasmas in porcine Mycoplasmal pneumonia [ J ]. Lett Appl Microbiol, 1997, 25(5) : 327-330.
  • 8Caron J, Ouardani M, Dea S. Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in pigs by PCR amplification of the P36 and P46 genes [ J ]. J Clin Microbiol, 2000 , 38(4) : 1390-1396.
  • 9Wallgren P, Beskow P, FeUstrom C, et al. Porcine lung lesions at slaughter and their correlation to the incidence of infections by Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae during the rearing period [ J ]. Zentralbl Veterinarmed, 1994, 41(7-8) : 441-452.
  • 10Pointon AM, Byrt D, Heap P. Effect of enzootic pneumonia of pigs on growth performance[J]. Aust Vet J, 1985, 62( 1 ) : 13-18.

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