委陵菜HPLC指纹图谱及多指标含量测定研究

HPLC fingerprint and multicomponents determination of Potentilla chinensis

目的建立委陵菜的指纹图谱及多指标含量测定方法,为委陵菜的质量标准提升提供实验依据。方法采用HPLC法,色谱柱Diamonsil C18(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸水为流动相,梯度洗脱;体积流量1.0 mL/min;柱温25℃;进样量10μL;检测波长260 nm;建立10批委陵菜的指纹图谱,用中药色谱指纹图谱相似度评价系统软件(2004版)进行评价,聚类分析将10批药材分为2类;建立同时测定委陵菜药材中没食子酸、槲皮素及山柰酚3种成分的含量测定方法。结果建立了委陵菜的HPLC指纹图谱,共标定了12个共有峰;没食子酸、槲皮素和山柰酚线性关系良好,相关系数(r)均大于0.999。精密度、稳定性、重复性的RSD均小于3%,平均回收率分别为97.44%、97.64%、99.19%。结论建立的委陵菜的指纹图谱及多指标含量测定方法专属性强、重复性好,能有效地控制委陵菜的内在质量,为提高委陵菜的质量评价方法提供参考。

Objective To establish the HPLC fingerprint and multicomponents determination of Potentillae Chinensis Herba, and provide a scientific basis for the improvement of its quality standards. Methods The separation was performed on a chromatographic Diamonsil C18 column(250 mm × 4.6 mm, 5 μm), with acetonitrile-0.1% phosphoric acid as the mobile phase for gradient elution. Volume flow rate was 1.0 mL/min. Column temperature was 25 ℃. Injection was 10 μL and the detection wavelength was 260 nm. The fingerprints of 10 batches of Potentillae Chinensis Herba were established and evaluated by the similarity evaluation system of TCM(version 2004 A), which were divided into two categories by clustering analysis. Meanwhile, the content of galic acid, quercetin, and kaempferol was determined. Results The fingerprint of Potentillae Chinensis Herba was established. There were 12 common peaks in the fingerprint. Galic acid, quercetin, and kaempferol were separated with good linearity relationships(r > 0.999). The average recovery rates of the investigated compounds were 97.44%, 97.64%, and 99.19%, respectively. Conclusion The established fingerprint and multicomponents determination method of Potentillae Chinensis Herba have strong specificity and good repeatability, which can effectively control the internal quality of Potentillae Chinensis Herba and provide reference for improving the quality evaluation method of Potentillae Chinensis Herba.