摘要
Objective Our study aimed to analyze the expression of miR-564 and TGF-β1 in cancer tissues and the serum of patients with radiation-induced lung injury,and to investigate the relationship between them and radiation-induced lung injury.Methods In situ hybridization and real-time fluorescence quantitative method were used to detect the expression of miR-564.Additionally,immunohistochemistry and enzyme-linked immunosorbent assay(ELISA)were performed to detect the expression of TGF-β1.Results The overall incidence of acute radiation pneumonia was 55.9%(100/179).The incidence of≥grade 2 radioactive pneumonia was 24.0%(43/179)and that of grade 1 was 31.8%(57/179).The expression of miR-564 in grade≥2 was slightly higher than that in patients without or with grade 1,but there was no statistical difference(P=0.86).The serum level and ratio of miR-564 in patients with grade≥2 were significantly higher than those without or with grade 1(P=0.005,P=0.025,respectively).The expression of TGF-β1 in grade≥2 was significantly higher than that of patients without or with grade 1(P=0.017).The serum levels of TGF-β1 in grade≥2 were significantly higher than those in patients without or with grade 1(P=0.038).Although the ratio of TGF-β1 in radiation pneumonia of grade≥2 was significantly higher than that of without or with grade 1,there was no significant difference(P=0.24).Moreover,patients with higher expression of miR-564 and lower expression of TGF-β1 had better prognosis.Conclusion MiR-564 and TGF-β1 are predictors of radiation-induced lung injury.Monitoring its changing trend can improve the accuracy of predicting radiation-induced lung injury.The levels and ratio of serum miR-564 and TGF-β1 in patients with radiation-induced lung injury are related to the severity of radiationinduced lung injury.
Objective Our study aimed to analyze the expression of mi R-564 and TGF-β1 in cancer tissues and the serum of patients with radiation-induced lung injury, and to investigate the relationship between them and radiation-induced lung injury. Methods In situ hybridization and real-time fluorescence quantitative method were used to detect the expression of mi R-564. Additionally, immunohistochemistry and enzyme-linked immunosorbent assay(ELISA) were performed to detect the expression of TGF-β1. Results The overall incidence of acute radiation pneumonia was 55.9%(100/179). The incidence of ≥ grade 2 radioactive pneumonia was 24.0%(43/179) and that of grade 1 was 31.8%(57/179). The expression of mi R-564 in grade ≥ 2 was slightly higher than that in patients without or with grade 1, but there was no statistical difference(P = 0.86). The serum level and ratio of mi R-564 in patients with grade ≥ 2 were significantly higher than those without or with grade 1(P = 0.005, P = 0.025, respectively). The expression of TGF-β1 in grade ≥ 2 was significantly higher than that of patients without or with grade 1(P = 0.017). The serum levels of TGF-β1 in grade ≥ 2 were significantly higher than those in patients without or with grade 1(P = 0.038). Although the ratio of TGF-β1 in radiation pneumonia of grade ≥ 2 was significantly higher than that of without or with grade 1, there was no significant difference(P = 0.24). Moreover, patients with higher expression of mi R-564 and lower expression of TGF-β1 had better prognosis.Conclusion Mi R-564 and TGF-β1 are predictors of radiation-induced lung injury. Monitoring its changing trend can improve the accuracy of predicting radiation-induced lung injury. The levels and ratio of serum mi R-564 and TGF-β1 in patients with radiation-induced lung injury are related to the severity of radiationinduced lung injury.
基金
Supported by grants from the Fundamental Research for South-Central University for Nationalities(No.PJS140011604)
Chen Xiaoping Foundation Development of Science and Technology of Hubei(No.CXPJJH11800004-015)
