大剂量维生素C在支气管哮喘大鼠肺部感染中的疗效及对肺功能的影响研究

Effect of high-dose vitamin C on pulmonary infection in rats with bronchial asthma and its effect on pulmonary function

目的探讨大剂量维生素C在支气管哮喘大鼠肺部感染中的疗效及对肺功能的影响。方法制作人工感染支气管哮喘肺部感染大鼠的模型,随机分为两组,各82只(排除2只),对照组使用小剂量维生素C治疗,研究组运用大剂量维生素C治疗。对比两组大鼠的治疗效果,血清因子血小板活化因子(Platelet-activating factor,PAF)、巨噬细胞炎性蛋白-1α(Macrophage inflammatory protein-1α,MIP-1α)、可溶性髓系细胞触发受体-1(Soluble triggering receptor expressed on myeloid cells-1,sTREM-1)、降钙素原(PCT)、心钠肽(Atrial natriuretic polypeptide,ANP)、抗氧化水平超氧化物歧化酶(Superoxide Dismutase,SOD)、丙二醛(Malondialdehyde,MDA)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)及肺功能一秒用力呼气容积(Forced expiratory volume in one second,FEV1)、一秒钟用力呼气量与用力肺活量比值(Forced expiratory volume in one second to forced vital capacity ratio,FEV1%)、一秒钟用力呼气容积占用力肺活量百分比(FEV1/FVC)以及6分钟步行试验(6-MinuteWalk Test,6MWT)的影响。结果研究组大鼠的治疗效果优于对照组(P<0.001);研究组大鼠的SOD为(351.25±50.75)NU/ml低于对照组(P<0.05),而MDA为(4.59±1.62)nmol/L高于对照组(P<0.05),但GSH-Px差异无统计学意义;研究组大鼠血清PAF、MIP-1α、sTREM-1、PCT分别为(133.87±785.15)mU/ml、(20.83±2.27)pg/ml、(85.42±7.92)pg/ml、(0.66±0.12)ng/ml均低于对照组(P<0.05),而ANP为(9.51±0.87)mg/L高于对照组(P<0.05);治疗前两组大鼠肺功能差异无统计学意义,治疗后研究组大鼠FEV1、FEV1百分比(FEV1%)、FEV1/FVC及6MWT分别为(2.85±0.63)L、(42.16±5.92)%、(69.74±7.84)%及(308.97±42.13)m高于对照组(P<0.05)。结论支气管哮喘大鼠肺部感染治疗过程中,大剂量维生素C的治疗效果较好,具有一定的临床价值。

OBJECTIVE To investigate the effect of high-dose vitamin C on lung infection in rats with bronchial asthma and its effect on lung function.METHODS A rat model of artificial infection of bronchial asthma with pulmonary infection was established and randomly divided into two groups,82 rats in each group(excluding 2 rats).The control group was treated with small dose of vitamin C,whereas the experimental group was treated with high doses of vitamin C.The therapeutic effects were compared between the two groups of rats.The effects on Platelet-Activating Factor(PAF),Macrophage inflammatory protein-1α(MIP-1α),Soluble triggering receptor expressed on myeloid cells-1(sTREM-1),procalcitonin(PCT),atrial natriuretic polypeptide(ANP),superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)and forced expiratory volume in one second(FEV1),forced expiratory volume in one second to forced vital capacity ratio(FEV1%),FEV1/FVC and 6-MinuteWalk Test(6 MWT)were compared between the two groups.RESULTS The therapeutic effect of the study group was significantly higher than that of the control group(P<0.001).The SOD level of the study group was(351.25±50.75)NU/ml,significantly lower than that of the control group(P<0.05),and the MDA level was(4.59±1.62)nmol/L,significantly higher than that of the control group(P<0.05),while the gsh-px level was not significantly different.Serum PAF,mip-1 prunus,strem-1 and PCT of the study group were(133.87±785.15)mU/ml,(20.83±2.27)pg/ml,(85.42±7.92)pg/ml and(0.66±0.12)ng/ml,significantly lower than those of the control group(P<0.05),and APN was(9.51±0.87)mg/L,significantly higher than that of the control group(P<0.05).Lung function had no significant differences between the two groups before treatment,The FEV1,FEV1%,FEV1/FVC and 6 MWT in the experimental group after treatment were(2.85±0.63)L,(42.16±5.92)%,(69.74±7.84)%and(308.97±42.13)m,significantly higher than those of the control group(P<0.05).CONCLUSION In the treatment of pulmonary infection in rats with bronchial asthma,the therapeutic effect of high-dose vitamin C is better,and it has certain clinical value.