环境因素与P21基因多态性在新疆哈萨克族食管鳞癌风险关系的研究

Study on the relationship between environmental factors and P21 gene polymorphism in Xinjiang Kazakh esophageal cancer

目的研究环境因素与P21基因3’UTR位点的基因多态性与新疆哈萨克族食管鳞癌(ESCC)发病风险的关系。方法收集经病理确诊的新疆哈萨克族ESCC患者86例(试验组)和同期未患食管癌的病例82例(对照组)。用限制性片段长度多态性聚合酶链反应技术法检测研究对象P213’UTR位点的单核苷酸多态性,并探讨环境因素与P21基因在新疆哈萨克族ESCC发病中的作用。结果试验组中P213’UTR位点的T/C基因型分布频率高于对照组(57.0%vs29.3%),差异有统计学意义(P<0.01)。与携带P213’UTR CC型的不饮酒患者相比,携带T/C型且有饮酒史者发生ESCC的风险显著增高(P<0.05,OR=2.531,95%CI=0.217~7.897);与携带P213’UTR CC型的不饮用河水患者相比,携带T/C基因型且常饮用河水的患者发生ESCC的风险显著增高(P<0.01,OR=3.685,95%CI=0.578~5.449)。结论P213’UTR位点基因多态性与大量饮酒、饮用河水的基因-环境交互作用可能与新疆哈萨克族ESCC高发显著相关。

Objective To study the relationship between environmental factors and the genetic polymorphism of the 3’UTR locus of P21 gene and the risk of esophageal squamous cell carcinoma(ESCC)in Kazakh people in Xinjiang province.Methods 86 cases with ESCC(treatment group)and 82 cases without ESCC(control group)were collected.Polymerase chain reaction technique with restriction fragment length polymorphism was used to detect the single nucleotide polymorphisms of P213'UTR in the study subject,and the roles of environmental factor and P21 gene in the incidence of ESCC in the Kazakh ethnic group in Xinjiang were investigated.Results The frequence of T/C genotype distribution at the P213'UTR locus in treatment group was higher than that in control group(57.0%vs 29.3%),and the difference was statistically significant(P<0.01).Compared with non-drinking patients with P213'UTR CC type,patients with T/C type and the history of alcohol consumption had a significantly higher risk of ESCC type,(P<0.05,OR=2.531,95%CI:0.217-7.897).Compared with non-drinking river water patients with P213'UTR CC type,patients with T/C gene type who often drink river water have a significantly higher risk of ESCC(P<0.01,OR=3.685,95%CI=0.578-5.449).Conclusion The gene polymorphism of P213'UTR locus and the gene-environment interaction of heavy drinking and drinking river water may be significantly correlated with the high incidence of ESCC in Kazakh ethnic group in Xinjiang.