HPV阳性患者行宫颈活检及免疫组化P16蛋白检测的临床意义分析

Clinical Significance of Cervical Biopsy and Immunohistochemical Detection of P16 Protein in HPV Positive Patients

目的探讨高危型人乳头瘤病毒(HPV)阳性患者行宫颈活检及免疫组化P16蛋白检测的临床意义。方法选择2018年1月至2019年3月在我院经第二代杂交捕获法(HC2)、薄层液基细胞学技术(TCT)联合检测确诊的135例高危型HPV阳性患者,均行免疫组化P16蛋白检测、宫颈活检。结果 135例患者经宫颈活检,高级别上皮病变者33例,低级别上皮病变者59例,炎症/萎缩43例;P16蛋白在高级别上皮病变、低级别上皮病变、炎症/萎缩阳性表达率分别为100.00%(33/33)、83.05%(49/59)、34.88%(15/43),不同级别病变中P16表达率相比,差异有统计学意义(P<0.05)。结论对HC2筛查高危型HPV阳性患者应及时实施病理活检,并联合免疫组化P16蛋白检测,以提高诊断正确率。

Objective To investigate the clinical significance of cervical biopsy and immunohistochemical detection of P16 protein in high-risk human papillomavirus(HPV) positive patients. Methods From January 2018 to March 2019, 135 high-risk HPV-positive patients diagnosed by combined detection of second-generation hybridization capture method(HC2) and thin-layer liquid-based cytology(TCT) in our hospital were examined by immunohistochemical P16 protein test and cervical biopsy. Results There were 33 cases of high-grade epithelial lesions, 59 cases of low-grade epithelial lesions and 43 cases of inflammation/atrophy in 135 patients. The positive expression rates of P16 protein in high-grade epithelial lesions, low-grade epithelial lesions and inflammation/atrophy were 100.00%(33/33), 83.05%(49/59), 34.88%(15/43), respectively. There were significant differences in the expression rates of P16 in different-grade lesions(P<0.05). Conclusion Pathological biopsy and immunohistochemical detection of P16 protein should be carried out in time for HC2 screening of high-risk HPV positive patients in order to improve the diagnostic accuracy.