针刺治疗大鼠酒精戒断焦虑征的作用机制

Effect of Acupuncture on Anxiety of Ethanol Withdrawal in Rats

目的:观察针刺对大鼠酒精戒断焦虑样症状的治疗作用,探究针刺治疗大鼠酒精戒断焦虑样症状的作用机制。方法:选取健康的SD大鼠,随机分组,分为空白组、模型组、三阴交组、足三里组、电针两穴(三阴交+足三里)组、针刺两穴(三阴交+足三里)组,连续腹腔注射酒精(ethanol,Eth)28 d,制备酒精成瘾模型,撤酒精3 d,制备酒精戒断模型,撤酒精期间分别针刺相应穴位进行治疗,针刺3 d,高架十字迷宫(EPM)测其焦虑行为,ELISA测海马中促肾上腺皮质激素释放激素、环磷酸腺苷浓度。结果:与空白组比较,酒精戒断大鼠停留在开放臂的次数和时间明显减少(P<0.05),海马中cAMP、CRH的浓度明显升高(P<0.01);与模型组相比较,电针两穴组和针刺两穴组在EPM开放臂的的停留次数、时间明显地增加(P<0.05),海马中cAMP、CRH的浓度明显降低(P<0.01),而单穴位组对各检测指标的作用效果不明显(P>0.05)。结论:针刺能缓解酒精戒断焦虑征大鼠的焦虑,其抗焦虑的作用机制可能与调节大鼠脑内海马组织中cAMP-CRH相互作用有关。

Objective:To observe the therapeutic effect of acupuncture on anxiety-like symptoms of alcohol withdrawal in rats,and to explore the mechanism of acupuncture treatment of anxiety-like symptoms of Ethanol withdrawal in rats.Methods:Healthy SD rats were randomly divided into the blank group,the model group,Sanyinjiao group,Zusanli group,Electro-acupuncture group of two-point,and acupuncture group of two-point.Ethanol was injected intraperitoneally for continuous 28 days to prepare the model of alcohol addiction.Stopping alcohol injection for 3 days to prepare the model of alcohol withdrawal.During the withdrawal of alcohol,the corresponding therapies were applied to corresponding points respectively for three days.The EPM test was used to measure the anxiety behavior.The concentrations of CRH and cAMP in the hippocampus were measured by ELISA method.Results:Compared to the rats in the blank group,the number and time of the alcohol withdrawal rats staying on the open arms were significantly reduced(P<0.05);the concentrations of cAMP and CRH in the hippocampus were significantly increased(P<0.01).Compared to the model group,the number and time of staying on the open arms were significantly increased in the electro-acupuncture group of two-point and in the acupuncture group of two-point group(P<0.05);the concentrations of cAMP and CRH in hippocampus were significantly decreased in the two groups(P<0.01).The effect of each single point group showed no statistical difference in each monitoring index before and after the intervention(P>0.05).Conclusion:Acupuncture can relieve the anxiety-like behavior of alcohol withdrawal rats,which mechanism may be related to regulating cAMP and CRH.