Ⅰ型鼠尾胶原三维培养模型对乳腺癌细胞耐药性的影响研究

The study of the effects of rat tail collagen Ⅰ three-dimensional culture on the drug resistance of breast cancer cells

目的探讨基质材料Ⅰ型鼠尾胶原三维(3D)培养对乳腺癌细胞MCF-7耐药性的影响。方法根据细胞培养方法的差异将细胞分为2D组及3DⅠ型鼠尾胶原培养组。CCK8实验检测MCF-7在2D和3D组中对顺铂和紫杉醇的药物敏感性;采用流式细胞术检测MCF-7在各组中的干性表面标记CD44^+/CD24^-细胞亚群比例及细胞周期分析;荧光定量PCR(qPCR)检测各组MCF-7的肿瘤细胞干性标记SOX2和KLF4的表达,以及耐药性相关ABC转运蛋白ABCC1和ABCB1的表达。结果与2D组相比较,MCF-7在3DⅠ型鼠尾胶原培养模型中的耐药性显著增强;同时,流式细胞结果显示3D组CD44^+/CD24^-亚群细胞比例高于2D组,且细胞周期出现G1期阻滞。qPCR结果显示较2D组细胞,3D胶原组细胞的干性相关指标SOX2和KLF4,耐药相关ABC转运蛋白ABCC1和ABCB1 mRNA的表达显著提高。结论Ⅰ型鼠尾胶原3D培养后乳腺癌细胞MCF-7干性增加,细胞周期G1期阻滞,耐药性增强。Ⅰ型鼠尾胶原可望作为新的3D药物筛选模型并用于抗耐药性肿瘤细胞意义重大。

Objective To investigate the effects of three-dimensional(3 D) rat tail collagen Ⅰ culture on the drug resistance of human breast cancer cell MCF-7, including the drug sensitivity, identification of stem cell surface markers, cell cycle, mRNA expressions of stem cell related genes and drug resistance related ABC transporters. Methods Two experimental groups were established as 2 D group and 3 D rat tail collagen Ⅰ group according to the culture methods. Cytotoxicity of anti-tumor drugs of Paclitaxel and Cisplatin in 2 D and 3 D cultures of MCF-7 cells was measured by CCK8 assay. CD44^+/CD24^-subtype cells and cell cycle were analyzed by flow cytometry. qPCR was used to determine the expressions of stem cell related genes SOX2 and KLF4, and drug resistance related ABC transporters ABCC1 and ABCB1. Results Compared to 2 D group, the drug resistance of MCF-7 cells in 3 D group enhanced significantly. Flow cytometric analysis results indicated that the percentage of CD44^+/CD24^-subtype cells was higher than that of 2 D group and cell cycle showed G1 phase arrest. The results of qPCR revealed that the expression levels of stem cell related genes SOX2 and KLF4, and the drug resistance related ABC transporters ABCC1 and ABCB1 were significantly unregulated. Conclusion MCF-7 cells cultured in 3 D rat tail collagen I showed an increase of drug resistance accompanied by the upregulation of cell stemness and G1 phase arrest. Rat tail collagen Ⅰ could be used as a new promising scaffold material for studies of drug screening and drug resistance of cancer cells.