七氟烷对乳腺癌BT549细胞侵袭、迁移及MMP-2、MMP-9表达的影响

The influence of sevoflurane on invasion,migration and expression of MMP-2,MMP-9 in breast cancer BT549 cells

目的:探讨七氟烷对乳腺癌BT549细胞侵袭、迁移及基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的影响。方法:乳腺癌BT549细胞用七氟烷处理后,细胞划痕实验检测细胞迁移,Transwell小室检测细胞侵袭,Western blot检测MMP-2、MMP-9蛋白表达。结果:七氟烷处理后的BT549细胞迁移率由(36.45±3.21)%降低至(14.68±1.52)%,侵袭细胞数目由(136.58±13.36)个减少到(79.95±14.25)个,MMP-2水平由0.48±0.04减少为0.14±0.03,MMP-9水平由0.96±0.07减少为0.22±0.05,两组比较差异均具有统计学意义(P<0.05)。结论:七氟烷能够抑制乳腺癌BT549细胞迁移和侵袭,降低细胞中MMP-2、MMP-9表达水平。

Objective:To investigate the effects of sevoflurane on the invasion,migration and the expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) of breast cancer BT549 cells.Methods:Breast cancer BT549 cells were treated with sevoflurane.Cell migration was detected by cell scratch test,Transwell chamber was used to detect cell invasion.Western blot was used to detect the expression of MMP-2 and MMP-9.Results:The cell migration rate of BT549 treated with sevoflurane decreased from(36.45±3.21)% to(14.68±1.52)%.The number of invasive cells decreased from(136.58±13.36) to(79.95±14.25).The levels of MMP-2 decreased from 0.48±0.04 to 0.14±0.03.The level of MMP-9 decreased from 0.96±0.07 to 0.22±0.05.There were significant differences between the two groups(P<0.05).Conclusion:Sevoflurane can inhibit the migration,invasion and reduce the expression of MMP-2 and MMP-9 of breast cancer BT549 cells.