Bcl-2基因多态性与食管和贲门癌的关联研究

The association of Bcl-2 gene polymorphism with the esophageal cancer and gastric cardia adenocarcinoma in Hebei Province

目的 探讨Bcl-2基因-938位点单核苷酸多态性与食管癌、贲门癌的关联.方法 采用病例对照的研究方法,于2007-2010年在河北医科大学第四医院门诊收集食管癌、贲门癌患者各145、169例为病例组,同期选取195例来自该地就诊的非肿瘤患者为对照组.采用问卷调查收集研究对象信息,采集食管、贲门病变组织,提取基因组DNA,并检测Bcl-2基因-938位点基因型;采用多因素logistic回归模型,分析Bcl-2基因-938位点CC基因型与EC、GCA间的关联,并采用似然比检验分析年龄、性别、吸烟、饮酒、上消化道家族史与Bcl-2基因-938位点CC基因型的交互作用.结果 食管和贲门癌组年龄分别为(56.3±8.3)、(57.1±8.4)岁,对照组年龄为(54.7±7.1)岁,差异均无统计学意义(P值均>0.05);食管组、贲门癌组Bcl-2基因-938位点CC基因型占比[48.3%(70/145)、48.5%(82/169)]均高于对照组[33.8%(66/195)](P值均<0.05).与携带AA基因型者相比,携带CC基因型者患食管癌、贲门癌的风险OR(95%CI)值分别2.386(1.195~4.764)和2.564(1.268~5.184).在携带CC基因型人群中,与家族史阳性、饮酒、男性相比,家族史阴性、不饮酒、女性患食管癌的风险更高;与不吸烟、家族史阴性、不饮酒、男性相比,吸烟、家族史阳性、饮酒、女性患贲门癌的风险更高(P交互<0.05).结论携带Bcl-2基因-938位点CC基因型者较易罹患食管癌、贲门癌.

Objective To investigate the association between the promoter region-938 polymorphism of B-cell lymphoma/leukemia-2 (Bcl-2) gene and the esophageal cancer (EC) and gastric cardia adenocarcinoma (GCA) in Hebei Province. Methods From 2007 to 2010, 145 esophageal cancer patients and 169 cardiaccancer patientsfrom the outpatient department of the Fourth Hospital of Hebei Medical Universitywereselected in a case group, and 195 non-tumor patients were selected in a control group during the same period. A questionnaire survey was used to collect information of research subjects. Pathological tissues were collected to extract genomic DNA and detect the genotype of bcl-2 gene-938. A multivariate logistic regression model was used to analyze the association between the bcl-2 gene locus 938 CC genotype and the EC and GCA. The interaction between age, gender, smoking, drinking, upper gastrointestinal family history and the bcl-2 gene locus 938 CC genotype was analyzed by likelihood ratio test. Results The age of the esophageal and cardiac cancer groups was (56.3±8.3) and (57.1±8.4) years old, and that of the control group was (54.7 ± 7.1) years old. The proportion of the bcl-2 gene locus 938 CC genotype in the esophageal group [48.3% (70/145) ] and the cardiac cancer group [48.5% (82/169) ] was higher than that in the control group [33.8% (66/195) ] (both P values<0.05).Compared with the AA genotype, the risk of esophageal cancer and cardiac cancerin people with the CC genotype was 2.386 (1.20-4.76) and 2.564 (1.27-5.18) respectively. In the population with CC genotype, compared with the positive family history, drinking, and male, the negative family history, non-drinking, and female had a higher risk of esophageal cancer;compared with the non-smoking, negative family history, non-drinking and male, the smoking, positive family history, drinking, and female had a higher risk of cardiac cancer (all the P interaction values were <0.05). Conclusion People with bcl-2 gene locus 938 CC genotype in Hebei Provincewere more likely to suffer from the esophageal and gastric cardia adenocarcinoma.