荸荠多糖中蛋白含量的测定

Determination of protein content in Eleocharis dulcis polysaccharides

荸荠是一种药食同源植物,为了建立荸荠多糖中蛋白含量的测定方法,采用水提醇沉法提取荸荠多糖,考马斯亮蓝法测定蛋白含量,比较了木瓜蛋白酶-Sevage法、Sevage法、三氯乙酸-正丁醇法脱蛋白的差异。结果表明,木瓜蛋白酶-Sevage法、Sevage法、三氯乙酸-正丁醇法的多糖损失率分别为21.92%、31.88%、47.05%,蛋白去除率分别为68.75%、47.08%、70.23%。考马斯亮蓝法适合荸荠多糖含量的测定,木瓜蛋白酶-Sevage法对于荸荠多糖是一种可行的脱蛋白方法。

To determine the protein content in polysaccharides from Eleocharis dulcis with multifunctions of both medicine and foods.The water extraction and alcohol precipitation method were adopted to obtain polysaccharide,Coomassie brilliant blue method was used to detect the protein content.The Sevage method,combined methods of papain and Sevage,combined methods of trichloroacetic acid and n-butanol were adopted to remove the protein respectively,and the effects of different methods were compared.The percentage of the removed protein for combined methods of papain and Sevage,Sevage method,combined methods of trichloroacetic acid and n-butanol were 68.75%,47.08%and 70.23%respectively,and the loss rate of polysaccharides were 21.92%,31.88%and 47.05%differently.Coomassie brilliant blue method was suitable for determination of protein in polysac-charides from Sevage.The combined methods of papain and Sevage was the best for removing protein for Eleocharis dulcis.