丹蒽醌对乳腺癌细胞MCF-7增殖及侵袭转移的抑制作用及其作用机制研究

Inhibitory effect of dantron on proliferation and invasion and metastasis of breast cancer cells MCF-7 and its mechanism

目的研究丹蒽醌对乳腺癌细胞MCF-7增殖及侵袭转移的抑制作用,并探讨其作用机制。方法 CCK-8法检测不同浓度(10、20、40、60、80、100μmol/L)的丹蒽醌作用24、48 h对乳腺癌细胞MCF-7增殖能力的影响;Transwell实验考察丹蒽醌对乳腺癌细胞MCF-7侵袭转移能力的影响。实时荧光当量PCR(RT-qPCR)法检测c-Myc、Cyclin D1 mRNA的表达水平;Western blotting法检测p-AMPKα、ACC、c-Myc、Cyclin D1蛋白的表达水平。结果不同浓度的丹蒽醌作用不同时间对乳腺癌细胞MCF-7增殖均具有抑制作用;20、40μmol/L的丹蒽醌能够明显的抑制乳腺癌细胞MCF-7的侵袭转移,同时能够激活p-AMPKα的表达,抑制ACC、c-Myc、Cyclin D1等基因的表达,从而抑制乳腺癌细胞MCF-7增殖。结论丹蒽醌对乳腺癌细胞MCF-7增殖及侵袭转移具有抑制作用,其作用机制可能与激活AMPK信号通路抑制c-Myc、Cyclin D1等增殖相关基因表达有关。

Objective To study the inhibitory effect of dantron on MCF-7 proliferation, invasion and metastasis of breast cancer cells, and to explore its mechanism. Methods CCK-8 assay was used to detect the effect of dantron at different concentrations(10,20, 40, 60, 80, 100 μmol/L) on MCF-7 proliferation of breast cancer cells for 24 and 48 h. Transwell assay investigated the effect of dantron on the invasion and metastasis of MCF-7 in breast cancer cells. Real-time fluorescence equivalent PCR(RT-qPCR) used to detect the expression levels of c-Myc、Cyclin D1 mRNA, and Western blotting were used to detect the protein expression levels of p-AMPKα, ACC, c-Myc and Cyclin D1. Results Different concentrations of dantron inhibited the proliferation of MCF-7 in breast cancer cells at different time, and 20, 40 μmol/L dantron can significantly inhibited the invasion and metastasis of MCF-7 in breast cancer cells, activate the expression of p-AMPKα, inhibited the expression of ACC, c-Myc, Cyclin D1 genes, and thereby inhibit the proliferation of MCF-7 in breast cancer cells. Conclusion Dantron can inhibit the proliferation, invasion and metastasis of MCF-7 in breast cancer cells, and its mechanism may be related to the activation of AMPK signaling pathway to inhibit the expression of cMyc, Cyclin D1 proliferation-related genes.