注射用重组人成纤维细胞生长因子-21皮下注射对大鼠呼吸系统的影响

Effect of subcutaneous injection of recombinant human fibroblast growth factor-21 on respiratory system in rats

目的应用全身体积描记法观察注射用重组人成纤维细胞生长因子-21(rhFGF-21)对大鼠呼吸系统的影响,确定药物可能关系到人的安全性的非期望出现的药物效应,为临床研究和安全用药提供参考。方法 SD大鼠40只,雌雄各半。实验设置4个组,分别为溶媒对照组、rhFGF-21 1.5、3.0、6.0 mg/kg剂量组,应用全身体积描记法采集清醒无束缚大鼠给药前、给药后0.25、1、2、4 h的呼吸功能指标。结果与溶媒对照组比较,各剂量组动物药后0.25、1、2、4 h均未见潮气量、每分钟通气量、呼吸频率出现统计学差异。结论大鼠单次皮下注射给予6.0 mg/kg的rhFGF-21,未见药物对大鼠呼吸系统指标产生明显影响。

Objective Reaserch on the effect of recombinant human fibroblast growth factor-21(rhFGF-21) on respiratory system in rats by whole body plethysmography, to determine the undesired drug effects that may be related to human safety, and provide reference for clinical research and safe medication. Methods 40 SD rats were used in this study, 20 males and 20 females. Four groups were set up, which were vehicle control group, rhFGF-21 1.5, 3.0 and 6.0 mg/kg dose groups. Respiratory function indexes of conscious and unconstrained rats before and 0.25, 1, 2 and 4 hours after administration were collected by whole body plethysmography. Results Compared with the vehicle control group, there was no statistical difference in tidal volume, minute ventilation, and respiratory rate at 0.25, 1, 2, and 4 h after treatment. Conclusion Rats were given a single subcutaneous injection of6.0 mg/kg rhFGF-21, there was no significant effect on the respiratory index in rats.