外周血血管生成素-2 mRNA定量检测与肝癌侵袭转移及预后的关系

Correlation between quantitative expression of Angiopoietin-2 mRNA in peripheral blood and the invasion/metastasis/prognosis in patients with hepatocellular carcinoma

[目的]检测原发性肝细胞性肝癌患者术前与术后外周血血管生成素-2(Angiopoietin-2,Ang-2)mRNA表达量的变化,探讨其与肝癌侵袭转移及预后的关系。[方法]应用实时荧光定量RT-PCR检测53例原发性肝细胞性肝癌患者术前及术后外周血Ang-2 mRNA表达量,术后随访3年,分析外周血Ang-2 mRNA表达量变化与肝癌侵袭转移及预后的关系。术前并与肝硬化组(30例)及正常对照组(25例)外周血Ang-2 mRNA表达量进行比较。[结果]肝癌组术前外周血Ang-2 mRNA表达量显著高于肝硬化组和正常对照组(P<0.01);肝癌组术后1个月时外周血Ang-2 mRNA表达量较术前显著下降(P<0.01)。术前外周血Ang-2 mRNA表达量与肿瘤大小、是否有血管侵犯、是否转移、血清AFP是否阳性、临床分期、是否术后复发均呈正相关(P<0.05或0.01),与性别、肿瘤包膜是否完整无相关性(P>0.05),与肝癌分化程度、术后生存期长短呈负相关(P<0.05或0.01)。多因素分析显示术前外周血Ang-2 mRNA表达量是影响肝癌患者预后的高危独立因素。[结论]术前外周血Ang-2 mRNA高表达促进肝癌侵袭转移,与不良预后相关,可能成为其术前及预后评价指标。

[Objective]To investigate the relationship between the quantitative expression of Angiopoietin-2(Ang-2) mRNA in peripheral blood and the invasion,metastasis and prognosis in patients with hepatocellular carcinoma.[Methods]The preoperative expression levels of Ang-2 mRNA in peripheral blood in 53 patients with hepatocellular carcinoma and 25 normal controls were measured by real-time quantitative RT-PCR respectively.The 3 years postoperative prognosis was followed-up.The relation between the preoperative expression levels of Ang-2 mRNA in peripheral blood and invasion and metastasis and prognosis in the patients with hepatocellular carcinoma was investigated.[Results]The expression levels of Ang-2 mRNA in peripheral blood in the patients with hepatocellular carcinoma were significantly higher than that in the normal controls(P<0.01).The preoperative expression levels of Ang-2 mRNA in peripheral blood in the patients with hepatocellular carcinoma were positively related with the tumor size,vascular invasion,metastasis,positive serum AFP,clinical stages,recurrence(P<0.05 or <0.01),but no relation with the sexuality and integrate tumor membrane(P>0.05),and were negatively related with the differentiated degree of hepatocellular tumor,postoperative survival time(P<0.05 or 0.01).Multivariate analysis revealed that preoperative expression levels of Ang-2 mRNA in peripheral blood were independent prognostic factor for the patients with hepatocellular carcinoma.[Conclusion]Preoperative high expression levels of Ang-2 mRNA in peripheral blood promote invasion and metastasis of hepatocellular carcinoma,are closely relative with the poor prognosis,may be a preoperative evaluation and prognostic factor for hepatocellular carcinoma.