摘要
Objective To clarify the biomarkers and molecular disorder mechanism of pathogenic unit of stasis-heat of acute intracerebral hemorrhage.Methods A total of 271 acute cerebral hemorrhage patients and their plasma samples were collected and classified into stasis-heat type and non-stasis-heat type according to Quantitative Diagnostic Scale of Stasis-heat Syndrome.Then 4 of each group were selected by 1∶1 paired design,and plasma samples of 4 pairs were collected or detecting differential proteins by tandem mass tag(TMT).The proteins expressed more than 1.3 times and P≤0.05 between the two groups were taken as differential proteins.The Ingenuity Pathway Analysis(IPA)bioinformatics software was performed in analyzing canonical pathways,upstream regulatory factor,molecular cellular functions,and protein interaction network of differential proteins.Results A total of 490 proteins were identified and quantified in each patient,of which 9 proteins were differentially expressed between the two groups(A1BG,ACTN1,CA1,CD44,CP,PRDX2,VCL,IgG H chain,and IGHV3-7),and A1BG,ACTN1,CA1,CD44,CP,PRDX2,and VCL were identified by IPA software.Compared with non-stasis-heat group,CD44 was down-regulated and the remaining six proteins were up-regulated.These proteins involved 5 canonical signaling pathways,2 upstream regulators and 5 kinds of molecules and cell functions.One associated functional network was generated with acceptable confidence.Conclusion Differential proteins reflecting the potential biomarkers of pathogenic unit of stasis-heat of the acute cerebral hemorrhage,are involved in the regulation of PI3K complexes,Akt and ERK-related cellular signaling pathways,which are closely related to inflammatory factors or their regulated upstream pathways.
