Experimental study of Chuyou Yuyang Granule on gastric ulcer model rats

Objective To study the healing effect of Chuyou Yuyang Granule(CYG)for the experimental gastric ulcer induced by acetic acid in rats.Methods Totally 50 SD rats were randomly divided into 5 groups,i.e.,normal group,model group,Western medicine treatment group,integrated Chinese and Western medicine group,and Chinese medicine treatment group,10 in each group.The rat model of gastric ulcer was established by means of glacial acetic acid corrosion method.The rats in each group were performed intragastric administration on the second day after the operation,Rats in the Western medicine group was administered omeprazole 0.132 mg/100 g by gastrogavage.In accordance with the intervention of Western medicine group,the integrated Chinese and Western medicine group added the CYG,and 0.5 mL/100 g was given for intragastric administration.The Chinese medicine group was given 0.5 mL/100 g CYG for intragastric administration,the normal group and the model model were given 1.25 mL/100 g physiological saline.The intervention was performed once a day for 14 consecutive days.The pathological changes of gastric mucosa in the rats of each group were observed,ulcer area and the ulcer inhibition rate of each treatment group was calculated.The changes of bFGF content in the serum samples were measured by ELISA method;the expression of bFGF protein in each group was detected by immunohistochemical method,and the expression of bFGF mRNA in each group was detected by Real-time PCR.Results Compared with the normal group,the gastric wall structure of the model group was destroyed,the mucous membrane was necrotic and defective,the gland disappeared,the cells were disarranged and the mucous layer was infiltrated by inflammatory cells;the expression of bFGF protein in the model group was relatively weak;the content of serum bFGF of the model rats decreased significantly(P<0.05),and the expression level of bFGF mRNA decreased(P<0.05).Compared with the model group,the gastric wall structure and mucous membrane recovery were better and the area of the ulcer of various drug intervention group was reduced(P<0.01,P<0.05)under the light microscope.Compared with the Western medicine group and the integrated traditional Chinese and Western medicine group,the inhibition rate of the Chinese medicine group was significantly increased(P<0.05).Compared with the model group,the expression level of bFGF protein in the gastric mucosa of the Chinese medicine group and the integrated Chinese and Western medicine group was stronger;The level of serum bFGF was higher in the integrated Chinese and Western medicine group,and was higher than that of the Chinese medicine group and the Western medicine group(P<0.05);the expression of bFGF mRNA in the Chinese medicine group and the Western medicine group increased,and was higher than that in the Western medicine group(P<0.05).Conclusion CYG could enhance the expression of bFGF protein and bFGF mRNA in gastric tissue of rats with gastric ulcer,regulate the growth of cell and differentiation,and promote the healing of ulcer.