Notch信号通路在急性移植物抗宿主病中的作用

The role of Notch pathway in acute graft versus host disease after hematopoietic stem cell transplantation

目的:分析异基因造血干细胞移植后患者Notch1的表达,了解Notch1信号通路与急性移植物抗宿主病发生、发展的关系。方法:采集54例异基因造血干细胞移植后患者外周血,同期采集26例自体造血干细胞移植患者和10例正常志愿者外周血标本作为对照,检测Notch1和Hes-1 mRNA的表达,分析其与急性移植物抗宿主病的关系。结果:异基因造血干细胞移植患者Notch1和Hes-1 mRNA表达较自体造血干细胞移植患者和正常志愿者高(P<0.05),其中急性移植物抗宿主病患者Notch1 mRNA表达明显高于非急性移植物抗宿主病患者、自体造血干细胞移植患者及正常志愿者(P<0.05)。Notch1 mRNA的表达与急性移植物抗宿主病总体分度呈正相关,急性移植物抗宿主病越严重,Notch1 mRNA的表达越高(P<0.05)。结论:Notch1信号参与调节急性移植物抗宿主病;Notch1及其靶基因的表达与急性移植物抗宿主病的严重程度密切相关。

Objective:To analyze the association between Notch signaling pathway and acute graft-versus-host-disease(aGVHD)in patients after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Method:Peripheral blood samples from 54 patients with various hematological disorders were collected after allo-HSCT.At the same time,peripheral blood samples from 26 patients with autogenic hematopoietic stem cell transplantation(auto-HSCT)and 10 healthy people were collected as controls.Notch1 and Hes-1 mRNA expressions were analyzed and compared among patients after allo-HSCT,auto-HSCT and healthy people.Result:Notch1 and Hes-1 mRNA expressions were significantly increased in allo-HSCT patients than those in auto-HSCT patients and healthy people(P<0.05).Notch1 mRNA expressions were higher in aGVHD patients than non-GVHD patients,auto-HSCT patients and healthy people(P<0.05).Notch1 mRNA expression was positivly correlated to the severity of aGVHD(P<0.05).Conclusion:Notch pathway plays an important role in regulating aGVHD.