miR-221增强乳腺癌细胞对多西他赛的耐药性

miR-221 enhances docetaxel resistance of breast cancer cells

目的:探究miR-221在人乳腺癌细胞T47D和MCF-7多西他赛(docetaxel)耐药性中的作用及机制。方法:qPCR检测多西他赛处理乳腺癌细胞T47D和MCF-7不同时间点,细胞中miR-221含量的变化;qPCR和Westernblot检测miR-221mimics的转染效率。用阴性对照(NC)或miR-221mimics转染细胞,不同浓度的多西他赛刺激细胞72小时后,MTT法检测细胞对多西他赛的耐药性;PI和AnnexinV双染法检测miR-221过表达对T47D和MCF-7细胞凋亡的影响;qPCR和Westernblot检测靶蛋白p27的表达。结果:在T47D和MCD-7中多西他赛刺激明显促进miR-221的表达量升高;miR-221在细胞内可以发挥生物学效应,降低靶蛋白p27的表达;且过表达miR-221明显增强T47D和MCF-7对多西他赛的耐药性,降低其凋亡率。结论:miR-221过表达可明显增强T47D和MCF-7细胞对多西他赛的耐药性。

Objective:To investigate the effects of miR-221 on docetaxel drug resistance of breast cancer cell line T47D and MCF-7.Methods:The relative expressions of miR-221 in T47D and MCF-7 cell lines treated with docetaxel or not were identified by qPCR.qPCR and Western blot were used to test miR-221 mimics transfection efficiency.MTT assay was used to test cell viability of cells treated with docetaxel or not in presence of different concentration of docetaxel.T47D and MCF-7 cells were treated with docetaxel,then flow cytometry was used to detect the effect of miR-221 on cell apoptosis.Results:The relative expression of miR-221 in T47D and MCF-7 cells treated with docetaxel was significantly higher than T47D cells.Compared with control cells,miR-221 mimics can significantly improve the proliferation of T47D cells.miR-221 mimics can also decrease the percentages of T47D cell apoptosis.Conclusion:miR-221 may significantly enhance breast cancer T47D and MCF-7 cells to docetaxel resistance.