Twist下调影响卵巢癌细胞株A2780顺铂敏感性的研究

Effect of Twist downregulation on cisplatin sensitivity of ovarian cancer cell line A2780

目的:探讨Twist对卵巢癌细胞株A2780顺铂敏感性及细胞增殖凋亡的影响。方法:通过Western blot筛选细胞株,构建小干扰RNA,qPCR筛选Twist-siRNA,将构建成功的Twist-siRNA逆转录于上皮性卵巢癌细胞株A2780中,通过CCK-8细胞术及流式细胞术,研究下调Twist对卵巢癌细胞株的增殖、凋亡及对化疗药物顺铂的影响。结果:选择人卵巢癌细胞株A2780,引入小干扰RNA后,Twist表达降低;细胞的增殖能力下降,凋亡增加(P<0.05),差异有统计学意义。si-Twist组相比对照组Twist基因的表达量明显降低,且呈现一定的顺铂浓度依懒性差异(P<0.05)。结论:下调Twist可抑制卵巢癌细胞A2780的增殖,促进其凋亡;下调Twist卵巢癌细胞A2780对顺铂的敏感性增加。

Objective:To investigate the effect of Twist on cisplatin sensitivity and cell proliferation and apoptosis of ovarian cancer cell line A2780.Methods:Western blot was used to screen cell lines and construct small interfering RNA.qPCR was used to screen Twist-siRNA.The constructed Twist-siRNA was retro-transcribed into epithelial ovarian cancer cell line A2780.Results:Human ovarian cancer cell line A2780 was selected,and Twist expression was decreased after small interfering RNA was introduced.The proliferation ability of cells decreased and apoptosis increased(P<0.05),and the difference was statistically significant.Conclusion:Down-regulation of Twist could inhibit the proliferation and promote the apoptosis of ovarian cancer cells A2780.The sensitivity of Twist ovarian cancer cells A2780 to cisplatin increased.