SSR标记在宁波地区水稻品种DNA指纹图谱构建及纯度鉴定中的应用

DNA Fingerprints Construction and Purity Identification Based on SSR Markers for Rice Varieties in Ningbo City

利用农业行业标准NY/T-1433-2007、NY/T-1433-2014中推荐的53对SSR引物,对宁波地区34个水稻品种进行DNA指纹图谱构建和遗传多样性分析。53对引物在研究材料中共获得183个等位基因,每对引物得到的等位基因数为1~7个,平均每对引物可获得3.5个等位基因。遗传相似性和聚类分析结果表明,34份水稻品种在遗传相似系数为0.5处可分为籼粳两个类群,其中,甬优系列籼粳杂交组合、6个常规粳稻及3个糯稻归为粳稻类群,6个常规籼稻和5个杂交籼稻归为籼稻类群。用RM590和RM3331对19个甬优杂交稻系列品种进行100粒种子的纯度鉴定,仅甬优2640和甬优50检测到1个单株的混杂。结果为SSR标记在宁波水稻品种的纯度和真伪鉴定方面的应用提供了一定的技术支撑。

The set of 53 SSR(Simple sequence repeat)markers for rice variety identification recommended in industrial standards of Ministry of Agriculture and Rual Affairs of the People's Republic of China,NY/T1433-2007 and NY/T1433-2014,were used to construct the DNA fingerprints and analyze the genetic diversity of 34 rice varieties in Ningbo city.A total of 183 alleles loci were detected among 34 varieties,the average number of detected alleles by each primer pair was 3.5 with a range from 1 to 7.The genetic similarity coefficient was calculated using the software NTSYS,and a dendrogram of genetic relationship was constructed using UPGMA method.The results showed that 34 cultivars were classified into two cluster groups with the similarity coefficient of 0.5,one group was composed of the Yongyou series hybrid rice combinations,6 conventional japonica rice varieties and 3 glutinous rice varieties,another group was composed of 6 conventional indica rice varieties and 5 indica hybrid rice combinations.Primers RM590 and RM3331 were used to SSR identification with 100 single seeds each hybrid rice of 19 Yongyou series,and the results showed that one contaminated plant was identified in Yongyou 2640 and Yongyou 50,respectively,no contaminated plants were identified in the other variaties.These results could provide technical support and theoretical basis for the application of SSR markers in rice variety and purity identification in Ningbo city.