IL-2(rs6822844）和IL-2Ra(rs2104286）单核苷酸多态性与原因不明复发性流产的相关性研究

Association between the Polymorphisms of IL-2( rs6822844 ) and IL-2Ra( rs2104286 ) SNPs and the Unexplained Recurrent Spontaneous Abortion

目的:探讨白细胞介素2基因(IL-2)及其受体α(IL-2Rα)单核苷酸多态性(SNPs)与原因不明复发性流产(URSA)的相关性。方法:采用病例对照研究,应用实时荧光定量聚合酶链反应(qRT-PCR)技术,对中国浙江地区145例URSA患者及220例健康对照IL-2(rs6822844)G/T及IL-2Rα(rs2104286)A/G进行分析。结果:URSA组IL-2Rα(rs2104286)AA基因型及A等位基因频率均高于对照组,差异均有统计学意义(P<0.05)。A等位基因携带者URSA患病风险是G等位基因携带者的2倍(OR=2.06,95%CI:1.34~3.16,P=0.001),AA基因型较AG+GG基因型URSA患病风险增加约1倍(OR=2.27,95%CI:1.40~3.68,P=0.001)。URSA组及对照组IL-2(rs6822844)GG基因型频率均为100%,差异无统计学意义(P>0.05)。结论:IL-2Rα(rs2104286)A等位基因可能是浙江地区URSA的遗传易感基因,IL-2(rs6822844)基因多态性与浙江地区URSA无相关性。

Objective:To investigate the association between the polymorphisms of single nucleotide polymorphisms(SNPs) of interleukin-2 gene(IL-2), IL-2 receptor α gene(IL-2 Rα) and the susceptibility to unexplained recurrent spontaneous abortion(URSA). Methods:Quantitative real-time polymerase chain reaction(qRT-PCR) was used to analysis SNPs of IL-2(rs6822844) G/T and IL-2 Rα(rs2104286) A/G in 145 URSA cases(URSA group) and 220 nomal controls(control group). Results:The frequencies of rs2104286 allele A and genotype A/A were higher in the URSA group when compared with the control group(P<0.05). The carriers of rs2104286 allele A increased two times of the risk of URSA(OR=2.06, 95%CI: 1.34~3.16, P=0.001), so did the rs2104286 genotype AA(OR=2.27, 95%CI: 1.40~3.68, P=0.001). The frequencies of rs6822844 genotype G/G in the two groups were all 100%(P >0.05). Conclusions:Allele A of IL-2 Rα(rs2104286) might confer the susceptibility to URSA in Zhejiang. IL-2(rs6822844) had no association with URSA.