蛋清多肽体内外抗氧化活性的研究

Antioxidant Activity of Egg White Polypeptide in Vitro/Vivo

本试验通过体外试验和动物试验两方面研究蛋清多肽(egg white polypeptide,EWP)的抗氧化能力,测定了1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picylhydrazyl,DPPH)自由基、羟自由基的清除能力和总还原能力以及小鼠血清、肝脏和心脏组织中超氧化物歧化酶(superoxide dismutase,SOD)活力、还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)含量。结果表明,蛋清多肽浓度为5 mg/mL时,其对DPPH自由基清除率为69.2%,羟自由基清除率为68.5%,蛋清多肽对DPPH自由基和羟自由基均有较好的清除作用,并且清除率与浓度呈正相关。蛋清多肽可显著提高小鼠血清、肝脏和心脏组织中SOD活力和GSH含量(P<0.05),显著降低机体内MDA含量(P<0.05),且蛋清多肽灌胃剂量为150~900 mg/kg时,抗氧化能力与蛋清多肽浓度有一定量效关系,且高剂量组效果最佳。本研究为开发新型生物活性肽,提高农产品附加值提供理论基础与数据支持。

In this study,the antioxidant capacity of egg white polypeptide(EWP)was studied in vitro and animal experiments,and the 1,1-diphenyl-2-picylhydrazyl(DPPH)free radical,hydroxyl free radical scavenging capacity and deoxidization capacity,as well as the superoxide dismutase(SOD)activity,glutathione(GSH)and malondialdehyde(MDA)contents in serum,liver and heart of mice were determined.The results showed that the egg white polypeptide significantly exhibited scavenging effect on DPPH radical and hydroxyl radical,which the DPPH free radical clearance rate was 69.2%and hydroxyl free radical clearance rate was 68.5%in the concentration of 5 mg/mL,and the scavenging rate was positively correlated with the concentration.Egg white polypeptide could significantly increase the SOD activity and GSH contents(P<0.05)and significantly reduce the MDA contents(P<0.05)in serum,liver and heart tissues of mice.In addition,egg white polypeptide improved antioxidant activity in a dose-dependent manner within the dosage of 150~900 mg/kg.This study provided theoretical basis and data support for the development of new bioactive peptides and the improvement of added value of agricultural products.