污水中絮凝细菌的分离及鉴定

Isolation and Identification of Flocculent Bacteria from the Sewage

为了阐明污泥中絮凝细菌的种类和特性,利用常规培养技术通过絮凝率测定和多次富集从污水水渠中分离和筛选絮凝菌,并对筛选得到的菌株进行形态学观察、生理生化特征分析、分子生物学鉴定以及絮凝率特性分析。结果利用营养琼脂培养基分离出絮凝菌20株,筛选出絮凝性能稳定且活性良好的两株微生物絮凝菌ZF1和ZF18,其絮凝率分别为57.68%和57.20%,超过其余18株的絮凝率。经鉴定可知,ZF1为志贺氏菌属(Shigella sp.),ZF18为荧光假单胞杆菌(Pseudomonas fluorescens)。此研究不仅为研究絮凝微生物提供良好的菌种资源,更对于研究污水治理具有很好的应用潜力。

To ascertain the varieties and characteristics of flocculent bacteria from the senage,conventional culturing techniques with flocculation rate determination and repeated enrichment were used to isolate and screen out flocculating bacteria from sewage drains.Besides,morphological observation,physiological and biochemical characteristics analysis,molecular biological identification and flocculation rate analysis were conducted on the screened bacteria.The results showed that 20 strains of flocculent bacteria were isolated by nutrient agar medium.The flocculent bacteria ZF1 and ZF18,which featured stable flocculating performance and good activity,were screened out with flocculating rate of 57.68%and 57.20%respectively,which were higher than that of the rest 18 strains.ZF1 was identified as Shigella sp.and ZF18 was identified as Pseudomonas fluorescens.Not only will the research project allow flocculating microorganism studies access to good bacteria resources,but also promise huge application potential to the studies on sewage treatment.