鹿尾蛋白与多肽制备工艺优化及其对TM3细胞增殖活性研究

Optimization of Preparation Processing of Deer Protein and Peptide and Its Proliferation Activity on TM3 Cells

以梅花鹿尾为研究对象,采用正交试验优化超声水提蛋白工艺及水酶法提取多肽工艺,比较两种最佳工艺所得物对小鼠睾丸间质细胞(TM3)增殖活性的影响。结果表明,超声水提蛋白工艺最佳条件为料液比1∶5 g/mL、超声功率200 W、提取时间1 h、提取温度40℃,在此条件下蛋白含量为60.72%±0.56%;水酶法提取多肽工艺中最佳蛋白酶为胰蛋白酶,酶解最佳条件为pH8、加酶量1500 U/g、酶解时间4 h、提取温度50℃,在此条件下水解度为22.85%±0.35%。水酶法提取鹿尾多肽对小鼠睾丸间质细胞(TM3)增殖活性优于超声水提鹿尾蛋白。

Taking the sika deer tail as research object,ultrasonic water extraction of protein technology and water enzymatic extraction of peptides were optimized by orthogonal test.The proliferation activity of TM3 cells was compared between the two optimally obtained proteins.The results showed that the optimal ultrasonic water extraction of protein process were as follows:The ratio of material to liquid was 1∶5 g/mL,the ultrasonic power was 200 W,the extraction time was 1 h,and the extraction temperature was 40℃,the content of protein was 60.72%±0.56%during the condition.The best protease of water enzymatic extraction of peptides was trypsin.The optimal conditions for enzymatic hydrolysis were pH8,enzyme amount addition 1500 U/g,enzymatic hydrolysis for 4 h at 50℃,and degree of hydrolysis was 22.85%±0.35%during the condition.The enzymatic extraction of deer tail polypeptide on the proliferation of TM3 was superior to that of ultrasonic water extracting deer tail protein.