唾液酸对急性酒精性肝损伤小鼠的抗氧化作用

Protective Effect of Sialic Acid on Acute Ethanol-induced Oxidative Damage in Mice

目的:探究唾液酸对急性酒精性肝损伤小鼠的保护作用。方法:将实验动物根据体重随机分配到空白对照组、模型对照组和唾液酸低、中、高剂量组(15、30、60 mg/kg·bw)。除空白对照组外,其他4个组在正常灌胃给药30 d后一次性经口灌胃50%乙醇,以建立急性酒精性肝损伤小鼠模型,选取血清和肝组织测定相应的指标(超氧化物歧化酶(Superoxide Dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)活力、谷胱甘肽(Glutathione,GSH)、丙二醛(Malondialdehyde,MDA)和肝组织蛋白质羰基(Protein Carbonyl,PC)含量)探究其抗氧化能力。结果:唾液酸可以显著降低酒精急性氧化损伤下机体的MDA和PC含量(P<0.05),并显著提高抗氧化酶GSH-Px、SOD活力和抗氧化物质GSH含量(P<0.05)。结论:唾液酸对小鼠的急性酒精氧化损伤具有一定的保护作用。

Objective:To explore the protective effect of sialic acid on acute ethanol-induced oxidative damage in mice. Method:The experimental animals were randomly divided into five groups control group,model group,low,medium and high dose sialic acid group(15,30 and 60 mg/kg·bw)according to the body weight. Except the control group,the other four groups were given 50% ethanol orally after 30 d administration to establish an ethanol-induced oxidative damage model. To study the antioxidant activity,serum and liver tissues were selected to detect the corresponding indicators,such as the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),the contents of glutathione(GSH),malondialdehyde(MDA)and protein carbonyl(PC). Results:The sialic acid could significantly reduce the contents of both MDA and PC(P<0.05),and significantly increase the activity of GSH-Px and SOD as well as the content of GSH(P<0.05). Conclusion:The sialic acid has a protective effect on acute oxidative damage of ethanol in mice.