摘要
目的探讨白芍总苷对局灶性脑缺血中枢海马CA1区病理改变的影响。方法通过阻断大脑中动脉制备局灶性脑缺血大鼠模型。白芍总苷低、中、高剂量组分别每日1次灌胃给予白芍总苷50、100、200 mg/kg,疗程28 d。红四氮唑(TTC)染色法计算脑梗死体积,干湿比重法计算脑组织含水量,苏木精-伊红(HE)法进行中枢海马CA1区病理学检查,原位末端转移酶标记染色法(TUNEL)观察CA1区神经元凋亡状况并计算凋亡指数(AI),透射电子显微镜(TEM)观察CA1区神经元超微结构变化。结果白芍总苷中、高剂量组能够明显降低局灶性脑缺血大鼠脑梗死体积(P<0.05或P<0.01),降低脑组织含水量(P<0.05),明显改善中枢海马CA1区组织细胞病理性改变,抑制CA1区神经元凋亡、AI显著降低(P<0.01),明显改善CA1区神经元细胞器等超微结构病理性改变。结论白芍总苷对局灶性脑缺血中枢海马CA1区病理改变及CA1区神经元超微结构病理改变具有保护作用。
Objective:To study the effect of Total Paeony Glucoside(TPG)on pathological changes of hippocam pal CA1 area in rats with focal cerebral ischemia.Methods:The model of focal cerebral ischemic was established by blocking the middle cerebral artery.TPG low,middle and high dose groups were given intraperitoneal adminis tration 50,100,200 mg/kg,once a day for 28 days.The water content of brain tissue was calculated with dry-wet specific gravity method;the volume of cerebral infarction was calculated with red tetrazolium(TTC)staining meth od,the pathological examination of central hippocampal CA1 area by HE staining;the neuronal apoptosis in CA1 area were observed by TUNEL staining and AI were calculated;the ultrastructural changes in neurons in CA1 re gion were observed by TEM.Results:TPG in middle and high dose groups could significantly reduce cerebral in farction volume(P<0.05 or P<0.01)and brain water content(P<0.05),improve the pathological changes of hip pocampal CA1 area,inhibit neuronal apoptosis and AI(P<0.01),and improve the ultrastructural pathological changes such as neuronal organelles.Conclusion:TPG has protective effects on the pathological changes of CA1 in hippocampus of central cerebral ischemia and the ultrastructural pathological changes of neurons in CA1 area.
作者
代小兰
李红霞
陈小丽
徐立
Dai Xiaolan;Li Hongxia;Chen Xiaoli(No.906 Hospital of Joint Services Force of PLA,Zhejiang,Ningbo 315000,China)
出处
《中国中医急症》
2019年第11期1942-1945,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
浙江省宁波市自然科学基金项目(2018A610415)
关键词
脑缺血
白芍总苷
海马CA1区
病理改变
超微结构
大鼠
Cerebral ischemic
Total paeony glucoside
Hippocampal CA1 region
Pathological change
Ultra structure
Rats