原儿茶酸可减轻退变软骨细胞的损伤

Protocatechuic acid alleviates degeneration of chondrocytes

背景:已有研究表明多种抗氧化剂由于对炎性因子存在抑制作用而表现出抗关节炎作用,但是原儿茶酸虽具有抗氧化和抗炎作用,但其是否也对骨关节炎有治疗作用,尚无相关报道。目的:探讨原儿茶酸在体外对白细胞介素1β诱导炎症的软骨细胞的生物学包括表型和细胞代谢的影响,为骨关节炎疾病提供一种潜在的药物保护手段。方法:收集乳鼠股骨软骨细胞,以10 mg/L的白细胞介素1β干预建立退变模型,以10,30和50 mg/L原儿茶酸对细胞进行治疗。实验于2017年10月经广西医科大学动物实验伦理委员会批准,批准号201710008。结果与结论:10-50 mg/L原儿茶酸可促进软骨细胞的增殖,其中30 mg/L原儿茶酸对关节软骨细胞的保护作用最强,对退变的关节软骨细胞具有促进增殖的作用,同时原儿茶酸有效促进软骨细胞生长,细胞外基质的合成和聚集蛋白聚糖、Ⅱ型胶原和Sox9的mRNA表达,同时下调炎症相关基因基质金属蛋白酶13的表达。表明原儿茶酸可促进软骨细胞增殖和维持软骨细胞表型,为进一步研究其在体内进行骨关节炎治疗和关节软骨退变损伤修复提供依据。

BACKGROUND:Preliminary studies have shown that numerous antioxidants exhibit antiarthritic effects due to their inhibition on inflammatory factors.With the antioxidant and anti-inflammatory abilities whether protocatechuic acid is effective in the treatment of osteoarthritis has never been reported.OBJECTIVE:To investigate the biological effect of protocatechuic acid on interleukin-1βinduced chondrocyte including phenotype and cellular metabolism in vitro,thereby providing a potential agent in osteoarthritis treatment,METHODS:The chondrocytes of neonatal rat femur were collected,intervened by 10 mg/L interleukin-1βto establish the degenerative model,and treated by 10,30 and 50 mg/L protocatechuic acid.The study was approved by the Laboratory Animal Ethical Committee of Guangxi Medical University in October 2017,with the approval No.201710008.RESULTS AND CONCLUSION:Protocatechuic acid effectively promoted chondrocyte growth in the range of 10-50 mg/L,while the dose of 30 mg/L was the strongest.Protocatechuic acid also enhanced the synthesis of the extracellular matrix and the mRNA expression of aggrecan,collagen II and Sox9,and downregulating the expression of the matrix metalloproteinase 13(a marker of inflammatory factor).To conclude,protocatechuic acid exerts a positive effect on the proliferation and phenotypic maintenance of articular chondrocytes,providing reference for its use in the treatment of osteoarthritis and repair of degenerative articular cartilage in vivo.