miR-195抑制舌鳞癌细胞系CAL27增殖、迁移和侵袭

miR-195 inhibits cell proliferation,migration and invasion of tongue squamous cell carcinoma cell line CAL27

目的研究miR-195对舌鳞癌CAL27细胞增殖、迁移和侵袭的影响及其机制。方法用RT-qPCR检测舌鳞癌组织及癌旁正常组织中miR-195 mRNA的表达;CAL27细胞的分组及处理:miR-195组(转染miR-195 mimics)、miR-con组(转染miR-NC)、si-con组(转染si-con)、si-激酶12抑制剂SUZ12组(转染si-SUZ12)、miR-195+Ctrl组(miR-195 mimics和pcDNA 3.1共转染)、miR-195+SUZ12组(miR-195 mimics和pcDNA 3.1-SUZ12共转染);MTT法检测细胞增殖;Transwell检测细胞迁移和侵袭;Western blot检测细胞SUZ12蛋白表达;双荧光素酶报告基因检测细胞荧光活性。结果舌鳞癌组织中miR-195 mRNA表达与癌旁正常组织及与健康口腔角质细胞NHOK相比显著降低(P<0.05);过表达miR-195及敲减SUZ12均可显著抑制CAL27细胞增殖、迁移及侵袭(P<0.05);过表达SUZ12可逆转miR-195对CAL27细胞增殖、迁移和侵袭的抑制。结论miR-195可抑制CAL27细胞的增殖、迁移和侵袭,其机制可能与靶向SUZ12有关。

Objective To study the effect of miR-195 on cell proliferation,migration and invasion of CAL27 cells,and to explore its mechanism.Methods RT-qPCR was used to detect the expression of miR-195 in tongue squamous cell carcinoma and adjacent normal tissues and cells.The miR-195 group(transfected with miR-195 mimics),miR-con group(transfected miR-NC),si-con group(transfected si-con),si-SUZ12 group(transfected si-SUZ12),miR-195+Ctrl group(co-transfected miR-195 mimics and pcDNA 3.1),miR-195+SUZ12 group(co-transfected miR-195 mimics and pcDNA 3.1-SUZ12);MTT assay was used to detect the proliferation in each group;Transwell was used to detect the migration and invasion in each group;Western blot was used to detect the protein expression of SUZ12 in each group;dual fluorescein report gene detection assay was used to detect the fluorescence from each group cells.Results Compared with normal tissues adjacent to the cancer,the expression of miR-195 was significantly decreased in tongue squamous carcinoma.Compared with human normal oral keratino-cytes NHOK,the expression of miR-195 was significantly decreased in tongue squamous carcinoma cells(P<0.05).Overexpression miR-195 and knockdown SUZ12 significantly inhibited the proliferation,migration and invasion of CAL27 cells;SUZ12 is the target of miR-195.Overexpression of SUZ12 reversed the inhibitory effect of miR-195 on proliferation,migration and invasion of CAL27 cells.Conclusions miR-195 can inhibit the proliferation,migration and invasion of CAL27 cell.The mechanism may be related to targeting at SUZ12,which could provide a new target for the prevention and treatment of tongue squamous cell carcinoma.