低聚果糖诱导的奶牛急性蹄叶炎血液糖脂代谢的研究

Metabolism of Glucose and Lipid in the Blood of Acute Laminitis in Dairy Cows Induced by Oligofructose

旨在探究奶牛急性蹄叶炎病程内血清生化指标的变化。本研究使用12头健康中国荷斯坦奶牛,实验组用17 g·kg^-1(按体重计量)的低聚果糖溶解在20 mL·kg^-1(按体重计量)的清水中灌服,对照组灌服等量清水,设定灌服时为0 h,分别在灌服前3 d至灌服后3 d期间固定时间段采血,检测血糖、胰岛素(INS)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、总胆固醇(CHOL)、瘦素(LEP)、脂联素(ADP)含量和CD36。结果:灌服后奶牛出现腹泻、跛行、蹄温升高、指(趾)动脉亢进、瘤胃蠕动减慢等症状。试验组血糖在灌服后6 h开始显著上升,持续到18 h,INS含量在6~12和36~48 h呈显著下降趋势。CHOL在灌服后6~72 h呈显著下降趋势,TG和HDL-C在12 h开始呈现显著下降趋势,LDL-C与对照组相比在36 h开始显著下降,LEP在12、18和36 h显著升高。血糖和INS水平,以及血清脂质水平变化明显,可能成为急性型蹄叶炎的诊断标准。

In order to investigate the changes in serum biochemical parameters during the course of acute laminitis in dairy cows, 12 healthy Chinese Holstein cows were used in this study. The experimental group was treated with 17 g·kg^-1 body weight of oligofructose which was dissolved in 20 mL·kg^-1 body weight of water, and the control group was given the same amount of water. Blood samples were collected at several fixed time points from 3 days before administration to 3 days after administration to detect blood glucose, insulin(INS), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), total cholesterol(CHOL), leptin(LEP), adiponectin(ADP) and CD36. Results were as follows: After the administration, the cows developed symptoms such as diarrhea, lameness, increased hoof temperature, hyperthyroidism of the finger(toe), and slowing of rumen peristalsis. The blood glucose of the experimental group began to rise significantly at 6 h after administration and lasted untill 18 h, and the INS content decreased significantly at 6-12 and 36-48 h. CHOL showed a significant downward trend from 6 to 72 h after administration. TG and HDL-C showed a significant downward trend at 12 h. LDL-C began to decrease significantly at 36 h compared with the control group. LEP was increased significantly at 12, 18 and 36 h. Blood glucose and INS levels, as well as serum lipid levels, are significant and may be the diagnostic criteria for acute laminitis.