亚临界条件下水相液化烟草工艺及产物的研究

Study on Technology and Products of Aqueous Liquefaction Tobacco under Subcritical Condition

以云南烟叶为原料,以水为溶剂,采用水热法制备液化物。以液化提取率为目标,对影响烟叶提取率的液料比、液化温度、液化时间三个影响因素进行了单因素实验,并在此基础上用正交实验来确定了烟叶最佳液化工艺。分别使用石油醚和二氯甲烷对液化物进行萃取,用气相色谱-质谱联用技术(GC-MS)对萃取成分进行分析。结果表明:烟叶的最优液化工艺是液料比为15∶1,液化温度为180℃,液化时间为2 h。影响烟草提取率的顺序为:液化温度>液化时间>液料比,液化温度对提取率有显著影响。石油醚萃取液中以烷烃类化合物为主,二氯甲烷萃取提取液得到的化合物成分较全。

Yunnan tobacco leaves were used as raw materials,and water was used as solvent to prepare liquefied products by hydrothermal method.With the goal of liquefaction extraction rate,single factor experiments were carried out on three influencing factors,liquid/material ratio,liquefaction temperature and liquefaction time,which affected the extraction rate of tobacco leaves.Based on this,orthogonal experiments were used to determine the optimal liquefaction process of tobacco leaves.The liquefied products were extracted using petroleum ether and dichloromethane,respectively,and the extracted components were analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that the optimum liquefaction process of tobacco leaves was as follow:the ratio of liquid to material was 15∶1,the liquefaction temperature was 180℃,and the liquefaction time was 2 h.The order of affecting the extraction rate of tobacco was as follow:liquefaction temperature>liquefaction time>liquid to material ratio,and liquefaction temperature had a significant effect on the extraction rate.Alkane compounds were the main components in petroleum ether extraction solution,and the dichloromethane extract had a relatively complete compound.