狂犬病病毒感染小鼠原代神经元细胞模型的建立

ESTABLISHMENT OF A MOUSE PRIMARY NEURONAL MODEL FOR RABIES VIRUS INFECTION

为了探究狂犬病病毒致神经系统症状的分子机制,本研究首先成功建立了高纯度胎鼠脑原代神经元细胞培养体系,进而通过使用实验室弱毒株SAD与固定毒株CVS11两种不同毒力的狂犬病病毒分别感染原代神经元细胞,评估这两种病毒在原代神经元细胞的最佳感染条件与病毒生长特性,建立不同毒力狂犬病病毒感染原代神经元细胞模型。在此基础上,通过Western blot技术检测狂犬病病毒糖蛋白、核蛋白以及神经囊泡循环的相关蛋白表达变化情况,结果显示固定毒株CVS11感染神经元细胞导致VAMP2蛋白表达量减少。本研究为进一步研究狂犬病病毒感染宿主致神经系统症状的机制奠定了研究基础。

To explore the molecular mechanisms of rabies virus-induced neurological symptoms,the highly pure primary neuronal cells were prepared from fetal mouse brain and infected with the attenuated SAD strain and fixed CVS11 strain.The optimal infection conditions and viral growth curves were determined afterwards.The expression of viral glycoprotein,nuclear protein and related other proteins in the vesicles were examined in Weston blotting.The results showed that the infection of neuronal cells by the fixed CVS11 strain induced the decrease of VAMP2 protein expression.The establishment of the primary neuronal cell model provided the foundation for further research on the mechanism of neurological symptoms caused by rabies virus infection.