枸杞提取物对骨质疏松症大鼠的改善作用

Effect of Chinese Wolfberry Extract on Osteoporosis Rats and Its Mechanism

为了探究枸杞提取物能够对骨质疏松症状起到改善效果,本文通过实验,根据枸杞提取物的浓度和各项指标增减变化,来探讨其对骨质疏松症大鼠症状的改善作用。选取80只符合实验要求的雌性大鼠平均分为两组,对其中一组进行去除卵巢手术,构建骨质疏松大鼠模型,采用水提法提取不同浓度的枸杞提取物,对各组大鼠实施不同浓度枸杞提取物和生理盐水的灌胃处理,70d后处死大鼠并检测各项相关指标。研究结果表明,枸杞提取物可有效提升骨质疏松大鼠骨密度约为0.05g/cm、转化生长因子-β1约为28.15μg/mL、一氧化氮约为7.05μmol/L、一氧化氮合酶约为8.23 U/mL、磷离子约为0.05 mmol/L以及骨钙素约为0.13 nmol/mL,且枸杞提取物浓度与各指标提升呈正向增长;枸杞提取物可有效降低骨质疏松大鼠血清中钙离子约为0.15 mmol/L、镁离子约为0.10mmol/L、碱性磷酸酶约为13.17 U/L以及免疫调节因子白细胞介素-6约为12.75μg/mL、肿瘤细胞坏死因子-α约为1.06μg/mL;且枸杞提取物浓度与各指标降低呈反向增长。说明枸杞提取物可有效调节骨质疏松大鼠身体中细胞因子,缓解大鼠骨分解情况,达到治疗骨质疏松目的。

In order to explore the improvement effect of Lycium barbarum extract on the symptoms of osteoporosis, this paper discussed the improvement effect of Lycium barbarum extract on the symptoms of osteoporosis in rats through experiments, according to the increase and decrease of the concentration and various indexes of Lycium barbarum extract. Eighty female rats meeting the requirements were divided into two groups, one group for removing ovarian surgery to build osteoporosis rats model. Different concentrations of Chinese wolfberry extract extracted with the use of water, and physiological saline were gavaged to rats, the related indicators were examined 70 d after the death of rat. The results showed that the Lycium barbarum extract could effectively improve the bone mineral density of osteoporosis rats up to 0.05 g/cm, conversion growth factor-beta 1 to 28.15 μg/mL, nitric oxide to 7.05 μmol/L, nitric oxide synthase to 8.23 U/mL, phosphorus ion to 0.05 mmol/L, and osteocalcin to 0.13 nmol/mL. Lycium barbarum extract can effectively reduce the serum calcium ion to 0.15 mmol/L, magnesium ion to 0.10 mmol/L, alkaline phosphatase to 13.17 U/L, immunoregulatory factor interleukin-6 to 12.75 ug/mL and tumor cell necrosis factf-alpha tot 1.06 ug/mL in rats with osteoporosis. Moreover, the concentration of Lycium barbarum extract increased inversely with the decrease of various indexes. It is concluded that Lycium barbarum extract can effectively regulate the cytokines in the body of osteoporotic rats, alleviate the osteolysis of rats, and achieve the goal of treating osteoporosis.