巨桉BRI1基因的克隆和表达分析

Gene Cloning and Expression Analysis of BRI1 in Eucalyptus grandis

为了解BRI1基因在巨桉中的功能,采用PCR技术克隆了EgrBRI1基因,分析了EgrBRI1的生物信息学和亚细胞定位,并对EgrBRI1基因响应激素和胁迫的差异表达进行了分析。结果表明,EgrBRI1基因全长3893 bp,编码1197个氨基酸。EgrBRI1蛋白稳定,空间结构复杂,存在3个motifs,主要定位于细胞膜。茉莉酸甲酯和油菜素内酯(BR)处理后,EgrBRI1基因在叶片中的表达上升,而水杨酸处理则没有明显的变化。盐胁迫和冷胁迫下,EgrBRI1基因表达表现为先下降后上升的趋势。因此,EgrBRI1基因能快速对外施激素做出响应,并在巨桉抗逆方面发挥重要作用,这可能是通过对BR信号的响应来实现的。

In order to understand the function of BRI1 gene in Eucalyptus grandis,EgrBRI1 gene was cloned,and the bioinformatics and subcellular localization of EgrBRI1 were analysis.Meanwhile,the expression of EgrBRI1 gene were analyzed after treated with hormones and stresses by qRT-RCR.The results showed that the length of EgrBRI1 gene was 3893 bp,encoding 1197 amino acids.EgrBRI1 protein was relatively stable with complex spatial structure,which contains three motifs.EgrBRI1 protein was mainly located in cell membrane.When treated with methyl jasmonate and brassinolide,the expression of EgrBRI1 gene in leaves showed an up-regulated trend with the time,while there was no significant change by treated with salicylic acid.Under salt and cold stresses,the expression of EgrBRI1 gene was down-regulated at first and then increased with the time.These indicated that EgrBRI1 gene could respond quickly to applied hormone and play an important role in stress resistance of E.grandis,which might be achieved by responding to BR signal.