18种黔产苦荞中微量元素与有效成分含量的相关性

Research on the Correlation of Trace Elements and Active Ingredients in F.esculentum from Guizhou Province

目的:测定18种黔不同产地苦荞中有效成分(芦丁、槲皮素、总黄酮)及13种微量元素[铅(Pb)、镉(Cd)、汞(Hg)、砷(As)、铜(Cu)、铝(Al)、锰(Mn)、锌(Zn)、铯(Se)、银(Ag)、锡(Sn)、锑(Sb)及钯(Ba)]的含量,探讨苦荞中有效成分与微量元素间的关系。方法:采用高效液相色谱法对黔产苦荞中芦丁和槲皮素进行含量测定,紫外分光光度法对总黄酮进行含量测定,微波消解-电感等离子体质谱法(ICP-MS)测定13种微量元素的含量;分析18批样品中微量元素与有效成分之间的相互关系。结果:18批苦荞样品的有害重金属元素(Pb、Cd、Hg、As、Cu)的残留量在标准限度浓度范围内,其中As含量与Cu、Al具有负相关性,与Ag具有正相关性;Cu含量与Al、Mn、Sb具有正相关性,与Ag具有负相关性;芦丁含量与Zn、Se具有正相关性,槲皮素含量与Zn、Se、Ag正相关,总黄酮含量与Zn正相关。结论:苦荞中芦丁、槲皮素、总黄酮与微量元素含量之间存在相关关系,可为苦荞质量的控制提供科学依据。

Objective:To determinate the contents of active components such as rutin,quercetin and total flavonoids,and 13 trace elements in F.esculentum from Guizhou province,discuss the relationships among its various constituents.Methods:Rutin and quercetin were determined by HPLC.The content of total flavonoids was determined using ultraviolet spectrophotometry.ICP-MS was employed to determine the contents of trace elements such as Pb and Cd.Relationship between mineral elements and effective components in F.esculentum was analyzed by double variable correlation method.Results:The residues of harmful heavy metal elements(Pb,Cd,Hg,As,Cu)in 18 batches of F.esculentum samples were within the standard limit concentration range.As had a negative correlation with Cu,Al and a positive correlation with Ag.Cu had a positive correlation with Al,Mn,Sb and a negative correlation with Ag.Rutin had a positive correlation with Zn,Se.Quercetin had a positive correlation with Zn,Se,Ag.There was a significant positive correlation between total flavonoids and Zn.Conclusion:Rutin,quercetin and total flavonoids contents are correlated with trace elements in F.esculentum,which provides a theoretical basis for quality control of F.esculentum.