4种长链非编码RNA在重度冠心病患者外周血单个核细胞中的表达及临床意义

Clinical Significance and Expression of Four IncRNAs in Peripheral Blood Mononuclear Cells of Patients with Severe Coronary Heart Disease

目的:探讨长链非编码RNA(lncRNA)肺腺癌转移相关转录本1(MALAT 1)、核糖核酸牛磺酸上调基因1(TUG 1)、Ezrin反义RNA1(EZR-AS 1)及lncRNA n342419(MANTIS)在重度冠心病(CHD)患者外周血单个核细胞(PBMC)中的差异表达及临床意义。方法:选取确诊为重度CHD患者35例作为CHD组,同期无CHD的体检人群38例为对照组;收集所有研究对象的临床特征资料,并抽取空腹静脉血8 mL,分别采用全自动生化分析仪和实时定量PCR(RT-qPCR)检测总胆固醇(CHOL)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)及4种lncRNA的表达,采用受试者工作特征(ROC)曲线分析4种lncRNA对重度CHD患者的诊断价值。结果:CHD组患者HDL-C水平比对照组体检人群明显降低(P<0.01),MALAT 1、TUG 1及EZR-AS 1的表达比对照组体检者明显升高,但MANTIS的表达则明显降低(P<0.01);ROC曲线结果显示,MALAT 1、TUG 1、EZR-AS 1、MANTIS的AUC分别为0.851(95%CI为0.764~0.938,P<0.01)、0.768(95%CI为0.662~0.875,P<0.01)、0.884(95%CI为0.811~0.957,P<0.01)及0.807(95%CI为0.707~0.907,P<0.01)。结论:MALAT 1、TUG 1、EZR-AS 1及MANTIS在重度CHD患者PBMC中差异性表达与CHD严重程度相关。

Objective:To investigate the expression and clinical significance of long noncoding RNA(lncRNA)metastasis-associated lung adenocarcinoma transcript 1(MALAT1),taurine up-regulated 1(TUG 1),Ezrin antisense RNA1(EZR-AS1),and lncRNA n342419(MANTIS)in peripheral blood mononuclear cells(PBMC)of patients with severe coronary heart disease(CHD).Methods:The experiment was divided into two groups,the control group(38 cases)and CHD group(diagnosed 35 cases).Collecting clinical data of all subjects and drawing 8ml venous blood on an empty stomach to analyze on automatic biochemical analyzer and qRT-PCR for following expressions of CHOL,HDL-C,LDL-C and four types of IncRNA.Adopting subjects ROC curve to analyze diagnostic value of four types of IncRNA on severe CHD patients.Results:Compared with the control group,the expression level of HDL-C of CHD was significantly lowered(P<0.01),MALAT1,TUG1,and EZR-AS1 increased significantly,while the expression of MANTIS decreased sharply(P<0.01).The results of ROC curve showed that the AUC of MALAT1,TUG1,EZR-AS1,and MANTIS were 0.0851(95%CI was 0.764~0.938,P<0.01),0.768(95%CI was 0.662~0.875,P<0.01),0.884(95%CI was 0.811~0.957,P<0.01),and 0.807(95%CI was 0.707~0.907,P<0.01),respectively.Conclusion:The differential expression of MALAT1,TUG1,EZR-AS1,and M ANTIS in PBMC of patients with severe CHD were closely related with severity of CHD and have the potential to be biomarkers for diagnosis and evaluation of CHD.