新疆产胡萝卜籽超临界CO2萃取物化学成分分析及其抗菌和抗氧化活性考察

Analysis of Chemical Constituents of Supercritical CO2 Extract from Xinjiang Daucus carota Seed and Its Antibacterial and Antioxidant Activities Study

目的:研究新疆产胡萝卜籽超临界CO2萃取物(简称“胡萝卜籽萃取物”)的化学成分,并初步考察其抗菌和抗氧化活性,为该植物相关产品的开发及质量控制提供参考。方法:利用超临界CO2萃取法萃取新疆产胡萝卜籽中的弱极性挥发性成分。采用气质联用技术并结合标准谱库对所得萃取物的化学成分进行鉴定,以峰面积归一化法计算各成分的相对百分含量。采用微量肉汤稀释法和琼脂培养基平板法考察胡萝卜籽萃取物对金黄色葡萄球菌、大肠杆菌、白色念珠菌的抗菌活性,同时采用薄层色谱-生物自显影法考察其抗氧化活性。结果:胡萝卜籽萃取物中共鉴定出20个化学成分(总相对百分含量为98.76%),主要成分为β-红没药烯、α-细辛脑、α-蒎烯、β-丁香烯、绵马酚等。胡萝卜籽萃取物对金黄色葡萄球菌、大肠杆菌、白色念珠菌均具有一定的抑制作用,其最低抑菌浓度分别为0.039、0.833、0.625 mg/mL,最低杀菌浓度分别为0.078、1.667、1.250 mg/mL;该萃取物对1,1-二苯基乙-三硝基苯肼(DPPH)自由基具有一定的清除能力。结论:超临界CO2萃取所得的新疆胡萝卜籽萃取物中含有复杂的单萜和倍半萜烯类化合物,并具有一定的抗菌和抗氧化活性。

OBJECTIVE:To study the chemical constituents of supercritical CO2 extract from Xinjiang Daucus carota seed(called“D.carota seed extract”for short),and to preliminarily evaluate its antimicrobial and antioxident activity,so as to provide reference for the development and quality control of related products of the plant.METHODS:The non-polar volatile part of Xinjiang D.carota seed was extracted by supercritical CO2 extraction method.The chemical constituents of the extract were identified and quantified by GC-MS and standard spectral labrary.The relative percentage content of each component was calculated by peak area normalization method.The antibacterial activity of D.carota seed extract against Staphylococcus aureus,Escherichia coli and Candida albicans were investigated by trace broth dilution method and AGAR medium plate method.Its antioxidant activity was investigated by TLC-bioautography method.RESULTS:Twenty chemical constituents were identified from D.carota seed extract(the total relative percentage content was 98.76%),mainly including β-bisabolene,α-asarone,α-pinene,β-caryophyllene,aspidinol,etc.D.carota seed extract has certain antimicrobial activity on S.aureus,E.coli and C.albicans.The minimum inhibitory concentrations were 0.039,0.833,0.625 mg/mL,and minimum bactericidal concentrations were 0.078,1.667,1.250 mg/mL,respectively.The extract has a certain scavenging ability to DPPH radical.CONCLUSIONS:D.carota seed extract obtained by supercritical CO2 extraction contains monoterpenes and sesquiterpenes,and possesses certain antibacterial and antioxidant activity.