结球甘蓝抗黑腐病基因SSR标记转CAPS标记

SSR Marker of Black Rot (Xanthommonas campestris pv. campestris) Resistance Gene of Cabbage (Brassica oleracea var. capitata) Turn CAPS Marker

以结球甘蓝抗病亲本材料A21与感病亲本材料D12杂交的杂种F1自交得F3为试验材料,采用分子标记的方法,研究课题组所设计的结球甘蓝抗黑腐病基因QTL连锁距离为5.1 cmol·L-1的OL9A03引物,经3次重复,SSR标记结果在785株中532株出现目的条带,253株未扩增出目的条带,再根据该标记测序,得到1条93 bp的序列,运用该序列设计共3对CAPS引物,其中2对CAPS引物均出现了目的条带,多态性消失。结果表明:SSR标记成功转化为序列特意扩展区域(CAPS)标记C001,用400株F3代群体进行黑腐病侵染验证,侵染植株中283株表现为抗性,117株表现为感病性,原SSR标记和新CAPS标记结果一致,可达到分子标记辅助育种的目的。

The hybrid F1 hybridized with the cabbage-resistant parent material A21 and the susceptible parent material D12 were used as the experimental material,and the QTL of the black rot resistance gene designed by the research team was studied by molecular marker method.The OL9 A03 primer with a linkage distance of 5.1 cmol·L-1 was subjected to 3 replicates.The SSR marker results showed that the target band was 532 in 785 strains,and the target band was not amplified in 253 strains.Then,according to the label,a 93 bp fragment was obtained.Sequence,a total of 3 pairs of CAPS primers were designed by using this sequence,and 2 pairs of CAPS primers showed a target band,and the polymorphism disappeared.The results showed that the SSR marker was successfully transformed into the sequence deliberately extended region(CAPS) marker C001,and 400 strains of F3 generation were used to verify the black rot infection.283 strains of the infected plants showed resistance and 117 strains showed pathogenicity.The results of original SSR marker and new CAPS marker are consistent and can be used for the purpose of molecular marker-assisted breeding.