DNA倍体联合高危型HPV在宫颈鳞癌患者淋巴结转移中的表达

Expression of DNA ploidy combined with high-risk HPV in lymph node metastasis of patients with cervical squamous cell carcinoma

目的探讨DNA倍体联合高危型HPV(HR-HPV)在宫颈鳞癌患者术后盆腔淋巴结微转移中的表达及意义。方法根据术后淋巴结常规病理学检查(HE染色)结果分为淋巴结转移组(实验1组)和淋巴结无转移组(实验2组)。采用DNA-AICM及导流杂交方法分别对两组进行DNA倍体及HR-HPV的检测;将HE染色结果与DNA异倍体、HR-HPV检测结果进行对比。结果 DNA异倍体及HR-HPV在实验1组中的阳性表达率分别为56.07%及50.47%,双阳性率为44.86%。二者在实验2组中的阳性表达率分别为17.49%及10.59%,双阳性率为4.93%;二者在转移阳性淋巴结中的阳性表达率及双阳性率均为100%。二者在转移阴性淋巴结中的阳性表达率分别为19.58%及12.42%,双阳性率为6.32%。结论 DNA倍体及HR-HPV有可能作为宫颈鳞癌盆腔淋巴结微转移的指标,二者联合检测可提高盆腔淋巴结微转移的检出率。

Objective To investigate the expression and significance of DNA ploidy combined with high-risk HPV(HR-HPV) in pelvic lymph node micrometastasis in patients with cervical squamous cell carcinoma after operation.Methods According to the results of routine pathological examination(HE staining) of lymph nodes after operation,the patients were divided into lymph node metastasis group and non-metastasis group.DNA ploidy and HR-HPV were detected by DNA-AICM and conduction hybridization,and the results of HE staining were compared with those of DNA aneuploidy and HR-HPV.Results The positive rates of DNA aneuploidy and HR-HPV in lymph node metastasis group were 50.47% and 48.60% respectively,and the double positive rate was 44.86%.The positive expression rates of DNA aneuploidy and HR-HPV in non-metastasis group was 18.97% and 11.08%,respectively,and the double positive rate was 4.92%.The positive expression rates and double positive rates of DNA aneuploidy and HR-HPV in metastatic positive lymph nodes were 100%.The positive expression rates of DNA aneuploidy and HR-HPV in metastatic negative lymph nodes were 19.58% and 12.42% respectively,and the double positive rate was 6.32%.Conclusion DNA ploidy and HR-HPV may be used as indicators of pelvic lymph node micrometastasis in cervical squamous cell carcinoma,and their combined detection can improve the detection rate of pelvic lymph node micrometastasis.