氧化锆改性磷酸镁骨水泥的生物力学性能体外初步测试

Prelimainary Biomechanical Test of Zirconia Modified Magnesium Phosphate Cement

目的:对氧化锆(ZrO2)改性磷酸镁骨水泥(MPC)进行初步的生物力学性能评估。方法:将质量分数分别为0%,5%,10%,15%及20%五个梯度的ZrO2与MPC混合(MPC,Z5MPC,Z10MPC,Z15MPC及Z20MPC)后,按固液比3 g/mL的比例与去离子水调和制成氧化锆-可注射骨水泥(ZMPC),分别对ZMPC的固化时间、抗压强度、细胞毒性、碱性磷酸酶活性、生物降解性能进行检测。结果:MPC,Z5MPC,Z10MPC,Z15MPC及Z20MPC的固化时间分别为(9.10±1.12),(9.62±0.78),(10.40±1.16),(10.83±1.06),(10.02±1.16)min,Z15MPC自固化时间相比MPC差异有统计学意义(P<0.05)。MPC,Z5MPC,Z10MPC,Z15MPC及Z20MPC抗压强度分别为(5.78±0.39),(6.27±0.48),(10.68±1.82),(23.98±0.85),(15.30±0.84)MPa,Z10MPC,Z15MPC及Z20MPC与MPC相比差异有统计学意义(P<0.01)。MPC,Z5MPC,Z10MPC,Z15MPC及Z20MPC相对增殖率(RGR)分别为(87.2±3.8)%,(83.8±4.5)%,(81.8±4.5)%,(81.6±4.5)%及(81.8±5.6)%,均大于80%,各组材料对成骨细胞MC3T3-E1细胞增殖影响差异无统计学意义(P>0.05),根据材料毒性评级标准均为Ⅰ级。比色法测定MPC,Z5MPC,Z10MPC,Z15MPC及Z20MPC碱性磷酸酶(ALP)活性分别为0.229±0.025,0.269±0.013,0.298±0.024,0.378±0.019,0.236±0.038,Z5MPC,Z10MPC及Z15MPC组较MPC组有较高的ALP活性,差异有统计学意义(P<0.05);Z20MPC组与MPC对照组之间ALP表达差异不明显,差异无统计学意义(P>0.05)。质量丢失率显示Z5MPC,Z10MPC,Z15MPC以及Z20MPC的降解速度与MPC组类似,各组间降解率近似,差异无统计学意义(P>0.05)。结论:随着ZrO2的加入,ZMPC的固化时间得以延缓,抗压强度也有所提高,各组材料也表现出良好的生物相容性及优良的生物降解性能,有望成为新型的骨组织修复材料。

Objective:To do a preliminary biomechanical evaluation of zirconia modified magnesium phosphate cement.Methods:The zirconia-injectable bone cement(ZMPC) was prepared by mixing ZrO2 with MPC according to the mass fraction of 0%,5%,10%,15%,20% of ZrO2(Named:MPC,Z5 MPC,Z10 MPC,Z15 MPC,Z20 MPC) at the solid to liquid ratio of 3 g/mL.Then the setting time,compressive strength,cytotoxicity,alkaline phosphatase activity and biodegradability were tested respectively.Results:The setting time of MPC,Z5 MPC,Z10 MPC,Z15 MPC,Z20 MPC were(9.10±1.12),(9.62±0.78),(10.40±1.16),(10.83±1.06),(10.02±1.16)min,respectively.And the difference between Z15 MPC and ZPC were statistically significant(P<0.05).The compressive strength of MPC,Z5 MPC,Z10 MPC,Z15 MPC,Z20 MPC were(5.78±0.39),(6.27±0.48),(10.68±1.82),(23.98±0.85),(15.30±0.84)MPa,respectively.There were significant differences between Z10 MPC,Z15 MPC,Z20 MPC and MPC(P<0.01).The relative proliferation rate of MPC,Z5 MPC,Z10 MPC,Z15 MPC,Z20 MPC were(87.2±3.8)%,(83.8±4.5)%,(81.8±4.5)%,(81.6±4.5)%,(81.8±5.6)%,respectively.There was no significant difference in the proliferation of osteoblasts MC3 T3-E1 between the three groups(P>0.05).According to the material toxicity rating criteria,they were all grade Ⅰ.The activity of alkaline phosphatase of MPC,Z5 MPC,Z10 MPC,Z15 MPC,Z20 MPC were 0.229±0.025,0.269±0.013,0.298±0.024,0.378±0.019,0.236±0.038,respectively.The ALP activity of Z5 MPC,Z10 MPC and Z15 MPC groups was higher than that of MPC group(P< 0.05).There was no significant difference in ALP expression between Z20 MPC group and MPC control group(P>0.05).The weight loss rate showed that the degradation rates of Z5 MPC,Z10 MPC,Z15 MPC and Z20 MPC were similar to those of the MPC group.The degradation rate between the groups was similar,and there was no statistically significant difference(P>0.05).Conclusion:With the addition of ZrO2,the setting time of ZMPC is delayed,and the compressive strength is also improved.The materials of each group also exhibit good biocompatibility and excellent biodegradability,and it is expected to become a new type of bone tissue repair material.