柴胡加龙骨牡蛎汤对大鼠创伤后应激障碍的预防作用

The preventive effects of Chaihu Jia Longgu Muli decoction on rats of post traumatic stress disorder

目的观察柴胡加龙骨牡蛎汤对大鼠创伤后应激障碍(Post traumatic stress disorder, PTSD)的预防作用。方法采用单次延长应激建立PTSD大鼠模型。40只大鼠随机分为正常组、模型组、柴胡加龙骨牡蛎汤组、氟西汀组。造模前2周每天灌胃给药1次,造模后静养1周,采用旷场实验和僵立行为测试评价大鼠的焦虑和恐惧行为学变化,ELISA法检测大鼠血清皮质酮浓度。结果较之于正常组,模型组大鼠旷场实验运动总距离(P<0.05)、穿格次数(P<0.05)及单次最大运动距离减少(P<0.01),僵立行为次数和时间增加(P<0.05),血清皮质酮浓度升高(P<0.01)。较之于模型组,柴胡加龙骨牡蛎汤组旷场实验运动总距离增加(P<0.05),血清皮质酮浓度减低(P<0.05);氟西汀组僵立行为次数(P<0.01)和时间减少(P<0.05)。结论柴胡加龙骨牡蛎汤能有效预防PTSD模型大鼠的焦虑样行为,增强大鼠对应激的适应能力。

Objective To investigate the preventive effects of Chaihu Jia Longgu Muli decoction on rats of post traumatic stress disorder(PTSD). Methods Single prolonged stress(SPS) was used to establish PTSD rat model. 40 rats were randomly assigned to normal group, model group, Chaihu Jia Longgu Muli decoction group, and fluoxetine group. Drugs were given by intragastric administration once a day for 2 weeks before the model was established. After one week of rest, open field test and freezing behavior test were used to examine the anxiety and fear behaviors in rats. Serum corticosterone concentration was determined by enzyme linked immunosorbent assay.Results Compared with the normal group, rats in model group exhibited significant decrease in total distance of movement(P<0.05), crossing frequency(P<0.05), and single maximum distance of movement(P<0.01) in open field test, showed significant decrease in freezing behavior frequency and time(P<0.05), and had lower level of serum corticosterone(P<0.01). Compared with the model group, rats in Chaihu Jia Longgu Muli decoction showed significantly increase in total movement distance(P<0.05) in the open field tests and had lower level of serum corticosterone(P<0.05). Rats in the fluoxetine group showed significant decrease in freezing behavior frequency(P<0.01) and time(P<0.05).Conclusion In addition to enhance the adaptability to stress, Chaihu Jia Longgu Muli decoction is effective in preventing anxiety-like behaviors for rats with PTSD.