红花蜂花粉多糖组分(PBPC-Ⅱ)对人乳腺癌MDA-MB231细胞生长的影响及作用机制

Effect of PBPC-Ⅱ on the growth of Human Breast Cancer MDA-MB231 Cells and Related Mechanism

目的探究红花蜂花粉多糖组分Ⅱ(PBPC-Ⅱ)对人乳腺癌MDA-MB231细胞凋亡和生长的影响及作用机制。方法体外培养MDA-MB231细胞,对照孔不给药物,实验孔依次添加终浓度为0.12,0.24,0.48,0.96 mg/mL的PBPC-Ⅱ,处理时间分别为12,24,36 h, MTS法检测PBPC-Ⅱ对MDA-MB231细胞的增殖影响,且筛选出最适药物浓度;流式细胞实验检测细胞凋亡情况;qPCR及Western blot实验检测Bcl-2、Bax、P53、C-myc基因及蛋白表达水平变化。结果 PBPC-Ⅱ可促进MDA-MB231细胞的凋亡。qPCR及Western blot实验结果显示,PBPC-Ⅱ可在分子水平上,抑制C-myc、Bcl-2基因转录与蛋白表达,增强P53、Bax基因及蛋白的表达。结论 PBPC-Ⅱ可抑制MDA-MB231细胞的生长并加速凋亡,其作用机制可能与抑制细胞增殖,P53、Bax基因表达上调,下调C-myc、Bcl-2基因表达有关。

Objective To investigate the effects of PBPC-Ⅱ on the proliferation and apoptosis of human breast cancer MDA-MB231 cells and its mechanism.Methods MDA-MB231 cell cultured in vitro were treated with various final concentrations of PBPC-Ⅱ(0, 0.12, 0.24, 0.48, 0.96 mg/mL) for 12, 24 and 36 h. Cell proliferation was examined by MTS which could screen suitable drug concentration;MDA-MB231 cell apoptosis were analyzed by flow cytometry;Bcl-2、Bax、P53 and C-myc gene and protein expressions were examined by qPCR and Western blot. Results PBPC-Ⅱ could induced MDA-MB231 cell apoptosis. Western blot and qPCR showed that PBPC-Ⅱ could reduce the gene and protein expression of C-myc, Bcl-2 and elevate the gene and protein expression of P53, Bax on the molecular level. Conclusion PBPC-Ⅱ could inhibit the growth of MDA-MB231 cell and induce cell apoptosis. The mechanism might be related to inhibit cell proliferation, elevating the gene expression of P53 and Bax and reducing the gene expression of C-myc and Bcl-2.